CAJ | 학술논문

目的研究禽流感H5N1病毒感染BALB/c小鼠后对宿主细胞免疫功能和细胞因子水平变化的影响,探讨禽流感H5N1病毒感染哺乳动物的免疫发病机制.方法选用鹅源禽流感H5N1病毒感染BALB/c小鼠,采用流式细胞仪检测血液和脾脏T淋巴细胞及其亚群的变化,采用ELISA检测血液中细胞岗子(IFN-γ、TNF-α、IL-4、IL-18、IL-10、IL-2)及禽流感H5N1病毒特异性抗体的变化.结果禽流感H5N1病毒感染可引起对宿主短暂的、可恢复的细胞免疫功能损伤:血液CD3+、CD4+、CD8+ T淋巴细胞数量于染毒后第2～4天下降(第4天为最低值),脾脏T淋巴细胞数最于染毒后第5～8天下降(第6天为最低值),然后均逐渐恢复到正常水平.染毒后血液细胞因子变化表现为:血清IFN-γ、TNF-α水平下降,IL-4、IL-18、IL-10水平上升,IL-2水平无明显变化.从感染第7天开始检测H5N1禽流感特异性抗体为阳性,抗体水平逐渐升高至实验结束的感染第14天.结论 H5N1禽流感病毒感染可引起宿主T细胞免疫功能低下是其主要的免疫病理改变之一,细胞因子表达失平衡或过多的表达都可能对宿主产生免疫病理损伤.
목적 연구금류감H5N1병독감염BALB/c소서후대숙주세포면역공능화세포인자수평변화적영향,탐토금류감H5N1병독감염포유동물적면역발병궤제.방법 선용아원금류감H5N1병독감염BALB/c소서,채용류식세포의검측혈액화비장T림파세포급기아군적변화,채용ELISA검측혈액중세포강자(IFN-γ、TNF-α、IL-4、IL-18、IL-10、IL-2)급금류감H5N1병독특이성항체적변화.결과 금류감H5N1병독감염가인기대숙주단잠적、가회복적세포면역공능손상:혈액CD3+、CD4+、CD8+ T림파세포수량우염독후제2～4천하강(제4천위최저치),비장T림파세포수최우염독후제5～8천하강(제6천위최저치),연후균축점회복도정상수평.염독후혈액세포인자변화표현위:혈청IFN-γ、TNF-α수평하강,IL-4、IL-18、IL-10수평상승,IL-2수평무명현변화.종감염제7천개시검측H5N1금류감특이성항체위양성,항체수평축점승고지실험결속적감염제14천.결론 H5N1금류감병독감염가인기숙주T세포면역공능저하시기주요적면역병리개변지일,세포인자표체실평형혹과다적표체도가능대숙주산생면역병리손상.
Objective To study the cell immunity and eytokines responses to avian influenza A H5N1 virus infections in a BALB/c model to better understand the pathogenesis of H5N1 avian influenza disease. Methods Two hundred and twenty BALB/c mice of the infected group were inoculated with 0.1 ml (10-4.875 TCID50) of A/Goose/Guangdong/NH/2003 ( H5N1 ) virus intra-nasally. Fifty control mice received noninfectious allantoic fluid and another fifty control mice received normal sodium. Blood and spleen samples were collected from the live mice every 24 h during the 14 d post-infection. The changes of CD3 + T cells , CD4 + T cells, CD8 + T cells for cell immunity in blood circulation and spleen were detected by flow cytometry. And the cytokines and antibody responses in blood circulation were detected by ELISA. Necropsy was performed on mice that died during the experiment and those euthanized at end of study. Results Avian influenza A( H5N1) virus infections can make damages to the cell immune system transiently. The CD3 + T cells, CD4 + T cells, CDS + T cells declined at 24 days post infection in blood circulation and declined at 5-8 days in spleen, then recovered to the normal level gradually. The eytokines responses to the infections can be detected: the level of IFN-γ,TNF-α declined, IL-4, IL-18, IL-10 increased, and IL-2 changed little. The antibody increased rapidly from day 7 post infection until the end of the study (day 14 post infection). Conclusion Collectively, avian influenza A(H5N1) virus can cause cell immunity deficiency and an imbalance in the level of eytokines, which may contribute to the unusual severity of disease caused by the H5N1 avian influenza virus.