CAJ | 학술논문

目的进一步研究我国结膜吸吮线虫(Thelazia callipaeda)生活史及其中间宿主.方法采集自然感染的家蝇和冈田氏绕眼果蝇(Amiota okadai),检出线虫幼虫,分别接种动物;用结膜吸吮线虫初产蚴分别喂饲实验室繁殖的第二代家蝇和冈田氏绕眼果蝇.结果自流行区采集冈田氏绕眼果蝇493只,从其体内检获34条幼虫,接种2只家兔和1只家犬眼内,经18～44 d观察,获成虫11条.结膜吸吮线虫初产蚴在冈田氏绕眼果蝇消化道内脱鞘,钻过肠壁进入血腔,在雄蝇睾丸和雌蝇血腔膜形成虫泡囊.幼虫经2次蜕皮(需14～17 d)发育为感染性幼虫,然后经颈部移行至喙.当果蝇吸食人及犬、猫眼部分泌物时进入终宿主眼内,经2次蜕皮发育为成虫后,产出幼虫.成虫寿命可达30个月以上.结论我国结膜吸吮线虫的中间宿主为冈田氏绕眼果蝇.
목적진일보연구아국결막흡전선충(Thelazia callipaeda)생활사급기중간숙주.방법채집자연감염적가승화강전씨요안과승(Amiota okadai),검출선충유충,분별접충동물;용결막흡전선충초산유분별위사실험실번식적제이대가승화강전씨요안과승.결과자류행구채집강전씨요안과승493지,종기체내검획34조유충,접충2지가토화1지가견안내,경18～44 d관찰,획성충11조.결막흡전선충초산유재강전씨요안과승소화도내탈초,찬과장벽진입혈강,재웅승고환화자승혈강막형성충포낭.유충경2차세피(수14～17 d)발육위감염성유충,연후경경부이행지훼.당과승흡식인급견、묘안부분비물시진입종숙주안내,경2차세피발육위성충후,산출유충.성충수명가체30개월이상.결론아국결막흡전선충적중간숙주위강전씨요안과승.
Objective To further investigate the life cycle and intermediate host of Thelazia callipaeda in China.Methods Dogs and rabbits were experimentally infected with larvae of T. callipaeda from naturally infected houseflies Musca spp. and fruit flies Amiota okadai. Houseflies and A. okadai bred in laboratory were fed with newbom larvae of T. callipaeda to define the intermediate host of the eye worm. Results Two rabbits and one dog were infected with 34 larvae of T.callipaeda taken from 493 naturally infected A. okadai. As a eonsequence, 11 adult worms were harvested from the experimentally infected animals 18 to 44 days after infection. The development process of T. callipaecda larvae in A. ok adai included three successive stages. The infective larvae migrated through the hemocoel to the head and proboscis of A. okadai. Complete larval development in A. okadai required 14- 17 days under appropriate temperature. Infective larvae entered the conjunctiva sac of the definitive hosts (dogs, cats, and man) when infected A. okadai sucked their eye secretions. The larvae grew into adults, with two ecdyses in the process of development. The adult females began to produce newborn larvae in 35 days after infection. The longest life-span of T. callipaeda was more than 30 months. Conclusion A. okadai is the intermediate host of T. callipaeda in China.