CAJ | 학술논문

目的探讨内皮素(ET)在哮喘发病过程中的作用及其与肺功能的相关性。方法用高渗盐水诱痰法收集25例轻、中度哮喘发作期患者，26例哮喘缓解期患者及30名健康者的痰标本，用放射免疫法测定其中ET的浓度。结果⑴FEV1/FVC发作组最低，缓解组次之，正常组最高，各组比较差异均有显著性(均P＜0.01)。⑵发作组和缓解组诱导痰中炎症细胞数均明显高于正常组(均P＜0.01)。⑶发作组诱导痰中ET浓度明显高于缓解组和正常组(均P＜0.01)。⑷发作组诱导痰中ET浓度与FEV1/FVC和氧分压均呈负相关(r分别为－0.762、－0.878)，而与巨噬细胞数呈正相关(r＝－0.653)。结论哮喘发作期和缓解期患者气道腔内均存在慢性非特异性炎症。ET可能参与了哮喘的病理进展过程，并在哮喘的气道炎症和气道重塑中起了重要作用；诱导痰中ET的浓度可较好地反映气道腔局部ET浓度的变化。
목적탐토내피소(ET)재효천발병과정중적작용급기여폐공능적상관성。방법용고삼염수유담법수집25례경、중도효천발작기환자，26례효천완해기환자급30명건강자적담표본，용방사면역법측정기중ET적농도。결과⑴FEV1/FVC발작조최저，완해조차지，정상조최고，각조비교차이균유현저성(균P＜0.01)。⑵발작조화완해조유도담중염증세포수균명현고우정상조(균P＜0.01)。⑶발작조유도담중ET농도명현고우완해조화정상조(균P＜0.01)。⑷발작조유도담중ET농도여FEV1/FVC화양분압균정부상관(r분별위－0.762、－0.878)，이여거서세포수정정상관(r＝－0.653)。결론효천발작기화완해기환자기도강내균존재만성비특이성염증。ET가능삼여료효천적병리진전과정，병재효천적기도염증화기도중소중기료중요작용；유도담중ET적농도가교호지반영기도강국부ET농도적변화。
Objective　To investigate the role of endothelin(ET) in the pathogenesis of asthma and analyze the relationship between level of endothelin in induced sputum and pulmonary function:forced expiratory volume/forced vital capacity(FEV1/FVc).Methods　Induced sputum was obtained by inhaling 5% saline.Endothelin level in induced sputum obtained from 25 patients with mild or moederate attacking asthma(Group Ⅰ),26 patients with stable asthma(Group Ⅱ) and 30 healthy controls(Group Ⅲ) were measured by radioimmunoassay.Results　⑴FEV1/FVC in Group Ⅱ was higher than those in Group Ⅰ(P＜0.01),and lower than those in Group Ⅲ(P＜0.01),respectively.⑵The number of all inflammatory cells and macrophages in induced sputum from both the Group Ⅰ and Group Ⅱ were higher than those from the Group Ⅲ(P＜0.01)，respectively.⑶The level of ET in induced sputum from Group Ⅰ was higher than those from both Group Ⅱ and Group Ⅲ(P＜0.01)，respectively.⑷The level of ET in induced sputum from Group Ⅰ was negatively correlated with FEV1/FVC and oxygen partial pressure(PaO2),and positively correlated with the number of macrophages.Conclusions　There is non-specific chronic airway inflammation in patients with asthma.Endothelin may be involved in the pathological process of asthma,especially in the airway inflammation and its remodeling.The level of ET in induced sputum can reflect the level of ET in airway.