西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2009年
6期
754-758
,共5页
非酒精性脂肪性肝炎%慈菇消脂丸%环氧合酶-2%诱导型一氧化氮合酶%一氧化氮
非酒精性脂肪性肝炎%慈菇消脂汍%環氧閤酶-2%誘導型一氧化氮閤酶%一氧化氮
비주정성지방성간염%자고소지환%배양합매-2%유도형일양화담합매%일양화담
nonalcoholic steatohepatitis%Sagittara Sagittifolia L. Xiao-Zhi Pills%cyclooxygenase-2%inducible nitric oxide synthase%nitric oxide
目的 观察中药慈菇消脂丸对大鼠非酒精性脂肪性肝炎(NASH)的防治作用.方法 建立非酒精性脂肪性肝炎大鼠模型,随机分为正常组、模型组、慈菇消脂丸高剂量组、慈菇消脂丸低剂量组、东宝肝泰阳性药物对照组,观察肝组织病理形态学的变化,检测肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)的含量.免疫组化技术检测环氧合酶-2(COX-2)及诱导型一氧化氮合酶(iNOS)在肝脏的表达.结果 模型组大鼠肝组织出现脂肪变性及不同程度的炎性细胞浸润,镜下可见肝细胞内大量脂滴.与正常组相比较,模型组大鼠肝组织内SOD活性显著降低,MDA、NO含量显著提高,COX-2、iNOS蛋白表达显著提高,差异具有统计学意义;与模型组相比较,中药治疗组大鼠肝组织内SOD活性显著提高,MDA、 NO含量显著降低,COX-2、 iNOS蛋白表达显著降低,差异具有统计学意义;中药慈菇消脂丸高剂量组作用亦较阳性药物对照组显著.结论 慈菇消脂丸对大鼠非酒精性脂肪性肝炎具有明显的防治作用,其作用机制与改善肝组织脂肪变性程度、抑制脂质过氧化、抗炎有关.
目的 觀察中藥慈菇消脂汍對大鼠非酒精性脂肪性肝炎(NASH)的防治作用.方法 建立非酒精性脂肪性肝炎大鼠模型,隨機分為正常組、模型組、慈菇消脂汍高劑量組、慈菇消脂汍低劑量組、東寶肝泰暘性藥物對照組,觀察肝組織病理形態學的變化,檢測肝組織中丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)的含量.免疫組化技術檢測環氧閤酶-2(COX-2)及誘導型一氧化氮閤酶(iNOS)在肝髒的錶達.結果 模型組大鼠肝組織齣現脂肪變性及不同程度的炎性細胞浸潤,鏡下可見肝細胞內大量脂滴.與正常組相比較,模型組大鼠肝組織內SOD活性顯著降低,MDA、NO含量顯著提高,COX-2、iNOS蛋白錶達顯著提高,差異具有統計學意義;與模型組相比較,中藥治療組大鼠肝組織內SOD活性顯著提高,MDA、 NO含量顯著降低,COX-2、 iNOS蛋白錶達顯著降低,差異具有統計學意義;中藥慈菇消脂汍高劑量組作用亦較暘性藥物對照組顯著.結論 慈菇消脂汍對大鼠非酒精性脂肪性肝炎具有明顯的防治作用,其作用機製與改善肝組織脂肪變性程度、抑製脂質過氧化、抗炎有關.
목적 관찰중약자고소지환대대서비주정성지방성간염(NASH)적방치작용.방법 건립비주정성지방성간염대서모형,수궤분위정상조、모형조、자고소지환고제량조、자고소지환저제량조、동보간태양성약물대조조,관찰간조직병리형태학적변화,검측간조직중병이철(MDA)、초양화물기화매(SOD)、일양화담(NO)적함량.면역조화기술검측배양합매-2(COX-2)급유도형일양화담합매(iNOS)재간장적표체.결과 모형조대서간조직출현지방변성급불동정도적염성세포침윤,경하가견간세포내대량지적.여정상조상비교,모형조대서간조직내SOD활성현저강저,MDA、NO함량현저제고,COX-2、iNOS단백표체현저제고,차이구유통계학의의;여모형조상비교,중약치료조대서간조직내SOD활성현저제고,MDA、 NO함량현저강저,COX-2、 iNOS단백표체현저강저,차이구유통계학의의;중약자고소지환고제량조작용역교양성약물대조조현저.결론 자고소지환대대서비주정성지방성간염구유명현적방치작용,기작용궤제여개선간조직지방변성정도、억제지질과양화、항염유관.
Objective To observe the preventive and therapeutic effects of Sagittara Sagittifolia L. Xiao-Zhi Pills on nonalcoholic steatohepatitis (NASH) in rats. Methods Rat NASH models were established and the rats were divided randomly into five groups: the normal group, model group, high-dosage group and low-dosage groups of the treatment, and Dongbao Gantai positive drug group. Histopathological changes of the liver were observed. The levels of MDA, superoxide dismutase (SOD), and NO (nitric oxide) in the liver homogenate were determined. Expressions of cyclooxygenase-2 (cox-2) and inducible nitric oxide synthase (iNOS) in the liver were determined with immunohistochemical technique. Results In model group, extensive adipose degeneration and inflammatory cell infiltration were found in the liver. Compared with those in normal group, SOD activities in model group were obviously decreased (P<0.01), the contents of MDA and NO were obviously increased (P<0.01), and the expressions of cox-2 and iNOS proteins were obviously increased (P<0.01). Compared with those in model group, the activities of SOD in treatment groups were obviously increased (P<0.01), the contents of MDA and NO were obviously decreased (P<0.01), and the expressions of COX-2 and iNOS proteins were obviously decreased (P<0.01). The effect of high-dosage group was more obvious than that of Dongbao Gantai group (P<0.05). Conclusion Sagittara Sagittifolia L. Xiao-Zhi Pills can effectively prevent and treat nonalcoholic steatohepatitis in rats, and its mechanism may be associated with ameliorating hepatocellular steatosis, removing the free radicals and enhancing the anti-oxidation and anti-inflammation capability.
