CAJ | 학술논문

采用不连续垂直板聚丙烯酰胺凝胶电泳技术,对河曲藏獒、青海藏獒、青海藏狮犬和青海土种犬共4个群体103只犬的9个血液蛋白基因座(Tf、Po、Es-1、Es-2、Sα_2:、Hb、Alb、Pr、Amy)的多态性进行了检测,分析了两个藏獒群体的群体内和群体间的遗传变异,并以Nei氏标准遗传距离(D)为基础用UPGMA法探讨了不同犬群之间的遗传关系.结果表明,在4个被测犬群中,Tf、Po、Es-1、Es-2和Sα_2 5个基因座上存在多态性,其中Tf、Es-1和Po分别由3个等住基因所控制,Es-2和Sα_2,分别由2个等位基因所控制,而Hb、Alb、Pr和Amy基因座均呈现单态;河曲藏獒群体内遗传变异较青海藏獒丰富,而两个藏獒群体间的遗传分化程度很低(G_(ST)=0.0187);以Nei氏标准遗传距离(D)为基础的UPGMA法聚类结果表明.青海藏獒与青海藏狮犬和青海土种犬的遗传关系近于河曲藏獒.
채용불련속수직판취병희선알응효전영기술,대하곡장오、청해장오、청해장사견화청해토충견공4개군체103지견적9개혈액단백기인좌(Tf、Po、Es-1、Es-2、Sα_2:、Hb、Alb、Pr、Amy)적다태성진행료검측,분석료량개장오군체적군체내화군체간적유전변이,병이Nei씨표준유전거리(D)위기출용UPGMA법탐토료불동견군지간적유전관계.결과표명,재4개피측견군중,Tf、Po、Es-1、Es-2화Sα_2 5개기인좌상존재다태성,기중Tf、Es-1화Po분별유3개등주기인소공제,Es-2화Sα_2,분별유2개등위기인소공제,이Hb、Alb、Pr화Amy기인좌균정현단태;하곡장오군체내유전변이교청해장오봉부,이량개장오군체간적유전분화정도흔저(G_(ST)=0.0187);이Nei씨표준유전거리(D)위기출적UPGMA법취류결과표명.청해장오여청해장사견화청해토충견적유전관계근우하곡장오.
103 blood samples were taken from four populations of Hequ Tibetan mastiff,Qinghai Tibetan mastiff,Tibetan spaniel and native dogs of Qinghai.Nine blood protein loci(Tf,Po,Es-1,Es-2,Sα_2,Hb,Alb,Pr,Amy)were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system.Genetic variations were analyzed within and between two populations of Tibetan mastiff.The genetic relationship between dog populations were analyzed by Nei's standard genetic distance(D)and UPGMA.The results showed that genetic variations were observed in Tf,Po,Es-1,Es-2,and Sα_2,others were not polymorphic.There were three alleles at the loci of Tf,Es-1 and Po,two alleles at the loci of Es-2 and Sα_2.The genetic diversities within population of Hequ Tibetan mastiff were richer than that of Qinghai Tibetan mastiff.There was less variation among Tibetan mastiff populations and the genetic differentiation Wag very limited between Tibetan mastiff populations(G_(ST)=0.0187);A dendrogram by Nei's standard genetic distance(D)and UPGMA showed that genetic relationship between Hequ Tibetan mastiff and Qinghai Tibetan mastiff Was distant.