CAJ | 학술논문

目的研究骨髓间充质干细胞(BMSC)对大鼠脑胶质瘤C6细胞增殖的影响,并且探讨其相关机制.方法提取SD大鼠BMSC,进行体外培养、扩增.应用MTF比色法检测不同浓度BMSC上清液对C6细胞系增殖的抑制作用.用Transwell小室将BMSC与C6细胞进行双层培养,以HE染色法检测C6细胞形态变化.用划痕实验检测细胞迁移情况.结果 MTF结果显示BMSC上清液对C6细胞有抑制作用,120 h后1∶8、1∶4、1∶2的BMSC上清稀释液、BMSC上清原液培养组的生长抑率分别为17.1%、26.0%、39.9%、43.1%;与BMSC进行双层培养后的C6细胞其形态发生了明显改变,由圆形或多角形变为长梭形,细胞包体伸出长突起;划痕实验显示在36 h时对照组迁移率为100%,划痕完全愈合,而BMSC上清组划痕未见全愈合,迁移率为82%.结论 ①BMSC上清液能抑制胶质瘤C6细胞的恶性增殖,而且抑制效应呈剂量依赖性;②BMSC对C6的运动和迁移产生了抑制作用,从而降低了其恶性侵袭程度.
목적 연구골수간충질간세포(BMSC)대대서뇌효질류C6세포증식적영향,병차탐토기상관궤제.방법 제취SD대서BMSC,진행체외배양、확증.응용MTF비색법검측불동농도BMSC상청액대C6세포계증식적억제작용.용Transwell소실장BMSC여C6세포진행쌍층배양,이HE염색법검측C6세포형태변화.용화흔실험검측세포천이정황.결과 MTF결과현시BMSC상청액대C6세포유억제작용,120 h후1∶8、1∶4、1∶2적BMSC상청희석액、BMSC상청원액배양조적생장억솔분별위17.1%、26.0%、39.9%、43.1%;여BMSC진행쌍층배양후적C6세포기형태발생료명현개변,유원형혹다각형변위장사형,세포포체신출장돌기;화흔실험현시재36 h시대조조천이솔위100%,화흔완전유합,이BMSC상청조화흔미견전유합,천이솔위82%.결론 ①BMSC상청액능억제효질류C6세포적악성증식,이차억제효응정제량의뢰성;②BMSC대C6적운동화천이산생료억제작용,종이강저료기악성침습정도.
Objective To investigate the effects of bone mesenchymal stem cells (BMSC) on the proliferation of brain glioma C6 cell line in vitro and to discuss the possible mechanism.Methods BMSCs were harvested from SD rats, cultured in vitro. MTT was performed to measure the inhibitory rate of proliferation of C6 cells cultured by BMSCs supernatant. In transwell co-culture system, BMSCs-induced effects upon morphology of C6 cells were determined by HE stain and scrape-wound migration assay.Results A dose-dependent inhibitive tendency of BMSCs supernatant upon proliferation of C6 was demonstrated by MTT assay. When cocultured with BMSCs, C6 cells changed from a cuboid shape with pleomorphic hyperchromatic nuclei and poorly defined cytoplasm, to a spindle shape with elongate processes and retracted borders. Scrape-wound migration by decrease in the cellular migration of C6 in BMSCs supernatant.