国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
4期
301-304
,共4页
杨璐%沈洪%王立新%林琳%周晓波%吴静%刘增巍%徐艺%赵崧%葛超%骆殊%刘亚军
楊璐%瀋洪%王立新%林琳%週曉波%吳靜%劉增巍%徐藝%趙崧%葛超%駱殊%劉亞軍
양로%침홍%왕립신%림림%주효파%오정%류증외%서예%조숭%갈초%락수%류아군
树突状细胞%黄芪甲苷%胃癌%抗肿瘤免疫
樹突狀細胞%黃芪甲苷%胃癌%抗腫瘤免疫
수돌상세포%황기갑감%위암%항종류면역
Dendritic cell%Astragaloside Ⅳ%Gastric carcinoma%Antitumor immunity
目的 通过体外实验观察中药单体黄芪甲苷(astragaloside Ⅳ,AⅣ)联合树突状细胞(DC)瘤苗对机体免疫功能的调节作用.方法 外周血单个核细胞体外诱导、培养,负载胃痛SGC7901冻融抗原.制备DC瘤苗,分别观察AⅣ联合DC瘤苗、DC瘤苗、AⅣ淋巴细胞增殖情况及其激活的效应细胞在体外杀伤胃癌细胞的活性.结果 ①AⅣ联合DC瘤苗(联合)组、DC瘤苗组T淋巴细胞增殖率显著高于AⅣ组、T细胞组(阴性对照组)(P=0.000),其中联合组促淋巴细胞增殖能力优于DC瘤苗组(S/R为1∶5、1∶10、1:50、1∶100时,P分别为0.013、0.014、0.017、0.019);AⅣ组T淋巴细胞增殖与T细胞组比较,差异无统计学意义(P=0.185).②联合组及DC癌苗组所活化的T细胞对胃癌SGC7901细胞的杀伤率高于AⅣ组、未活化T细胞组(P=0.000),联合组杀伤能力优于DC瘤苗组(E/T为5∶1、10∶1、20∶1、50∶1时,P分别为0.023、0.012、0.016、0.011);而AⅣ组与未活化T细胞组几乎无杀伤作用(P=0.267).结论 AⅣ可通过DC瘤苗促进T淋巴细胞的增殖及杀伤肿瘤的活性,从而更有效地诱导抗胃癌特异性免疫应答.
目的 通過體外實驗觀察中藥單體黃芪甲苷(astragaloside Ⅳ,AⅣ)聯閤樹突狀細胞(DC)瘤苗對機體免疫功能的調節作用.方法 外週血單箇覈細胞體外誘導、培養,負載胃痛SGC7901凍融抗原.製備DC瘤苗,分彆觀察AⅣ聯閤DC瘤苗、DC瘤苗、AⅣ淋巴細胞增殖情況及其激活的效應細胞在體外殺傷胃癌細胞的活性.結果 ①AⅣ聯閤DC瘤苗(聯閤)組、DC瘤苗組T淋巴細胞增殖率顯著高于AⅣ組、T細胞組(陰性對照組)(P=0.000),其中聯閤組促淋巴細胞增殖能力優于DC瘤苗組(S/R為1∶5、1∶10、1:50、1∶100時,P分彆為0.013、0.014、0.017、0.019);AⅣ組T淋巴細胞增殖與T細胞組比較,差異無統計學意義(P=0.185).②聯閤組及DC癌苗組所活化的T細胞對胃癌SGC7901細胞的殺傷率高于AⅣ組、未活化T細胞組(P=0.000),聯閤組殺傷能力優于DC瘤苗組(E/T為5∶1、10∶1、20∶1、50∶1時,P分彆為0.023、0.012、0.016、0.011);而AⅣ組與未活化T細胞組幾乎無殺傷作用(P=0.267).結論 AⅣ可通過DC瘤苗促進T淋巴細胞的增殖及殺傷腫瘤的活性,從而更有效地誘導抗胃癌特異性免疫應答.
목적 통과체외실험관찰중약단체황기갑감(astragaloside Ⅳ,AⅣ)연합수돌상세포(DC)류묘대궤체면역공능적조절작용.방법 외주혈단개핵세포체외유도、배양,부재위통SGC7901동융항원.제비DC류묘,분별관찰AⅣ연합DC류묘、DC류묘、AⅣ림파세포증식정황급기격활적효응세포재체외살상위암세포적활성.결과 ①AⅣ연합DC류묘(연합)조、DC류묘조T림파세포증식솔현저고우AⅣ조、T세포조(음성대조조)(P=0.000),기중연합조촉림파세포증식능력우우DC류묘조(S/R위1∶5、1∶10、1:50、1∶100시,P분별위0.013、0.014、0.017、0.019);AⅣ조T림파세포증식여T세포조비교,차이무통계학의의(P=0.185).②연합조급DC암묘조소활화적T세포대위암SGC7901세포적살상솔고우AⅣ조、미활화T세포조(P=0.000),연합조살상능력우우DC류묘조(E/T위5∶1、10∶1、20∶1、50∶1시,P분별위0.023、0.012、0.016、0.011);이AⅣ조여미활화T세포조궤호무살상작용(P=0.267).결론 AⅣ가통과DC류묘촉진T림파세포적증식급살상종류적활성,종이경유효지유도항위암특이성면역응답.
Objective Through experiments in vitro,we explored the role of Chinese medicine monomer-Astragaloside Ⅳ with DC vaccine in the body immunologic function.Methods Peripheral blood mononuclear cells (PBMCs)from healthy volunteers were isolated,cultured and generated in vitro,pulsed with tumor antigen from SGC7901 gastric carcinoma cell lysates,produced DC vaccine.Observe T-cell proliferation responses stimulated by DC vaccine with AⅣ group,DC vaccine group and AⅣ group respectively,and the anti-tumor effects on SGC7901 cells in vitro.Results ①The T-cell proliferation rate of DC vaccine with AⅣ group and DC vaccine group were significantly higher than AⅣ group and T-cell group(negative control group)(P=0.000).The stimulating efficacy on T-cell proliferation of DC vaccine with AⅣ group was higher than that of DC vaccine group(S/R 1∶5,1∶10,1∶50,1∶100,P=0.013,0.014,0.017,0.019).Compared with T-cell group,the T-cell proliferation rate of AⅣ group had no statistically significance(P=0.185).②The killing rate of effector cells actived by DC vaccine with AⅣ group and DC vaccine group against SGC7901 gastric carcinoma cells were higher than that of AⅣ group and T-cell group(P=0.000).The Killing power of DC vaccine with AⅣ group was stronger than that of DC vaccine(E/T 5∶1,10∶1,20∶1,50∶1,P=0.023,0.012,0.016,0.011);while the group of AⅣ group and T-cell group cannot killing tumor cells.Both had no statistically significance(P=0.267).Conclusion AⅣ can stimulate T-cell proliferation and enhance the activity of killing tumor cells by DC,which induced specific antitumor response against stomach carcinoma cells effectively.
