CAJ | 학술논문

目的探讨PCR检测大鼠血液中细菌DNA对结肠吻合口漏的早期诊断价值.方法健康Wistar雌性大鼠48只,随机分成:A组(n=8)为假手术组;B组(n=20)为结肠-结肠吻合组;C组(n=20)为结肠吻合口漏组;采集手术前后外周血,抽提DNA.比较PCR检测大肠杆菌lacZ基因和细菌共有的16SrRNA基因的PCR阳性率,并观察各组的病理学情况.结果 C组外周血lacZ基因的PCR阳性率显著高于B组(P<0.05);C组外周血16SrRNA基因的PCR阳性率与B组差异无统计学意义(P>0.05).结论 PCR检测术后外周血lacZ基因对结肠吻合口漏的诊断可能有一定的意义,而检测术后外周血16SrRNA基因对结肠吻合口漏的意义可能不大.
목적 탐토PCR검측대서혈액중세균DNA대결장문합구루적조기진단개치.방법 건강Wistar자성대서48지,수궤분성:A조(n=8)위가수술조;B조(n=20)위결장-결장문합조;C조(n=20)위결장문합구루조;채집수술전후외주혈,추제DNA.비교PCR검측대장간균lacZ기인화세균공유적16SrRNA기인적PCR양성솔,병관찰각조적병이학정황.결과 C조외주혈lacZ기인적PCR양성솔현저고우B조(P<0.05);C조외주혈16SrRNA기인적PCR양성솔여B조차이무통계학의의(P>0.05).결론 PCR검측술후외주혈lacZ기인대결장문합구루적진단가능유일정적의의,이검측술후외주혈16SrRNA기인대결장문합구루적의의가능불대.
Objective To asses the value of detecting bacterial DNAs in rat's blood with PCR for early diagnosis of colonic anastemotic leakage.Methods 48 healthy female Wistar rats were random divided into three groups: Group A(n=8,sham operation group),Group B(n=20,colonic anastomosis group),Group C(n=20,colonic anastomotic leakage group).Group B and C rats underwent standardized colon resection 3cm away from the ileocecal junction 10cm,Group B rats were done with a complete anastomosis(end-to-end single layer anastomosis with 0# silk sutures) while Group C rats were done with an anastomosis leaving a 5mm opening in colonic anterior wall.lml and 3ml venous blood samples were collected from Group A,B and C.DNAs were extracted from these blood samples and PCR techniques were used to amplify lacZ genes from Escherichia coli and 16S ribosomal RNA genes(16SrRNA genes) 3 days after operation.The data were analyzed by chi square test.Specimens of the experimental intestine were HE stained for pathological studies.Results The positive ratios of expressing lacZ genes in peripheral blood(PB) with PCR in Group C were significantly higher than that in Group B(P<0.05),hut there were no differences between the two groups in expressing 16SrRNA genes(P>0.05).Conclusions It could be a useful way to detect lacZ genes of Escherichia coli from PB by PCR but not 16SrRNA genes for diagnosis of colonic anastomotic leakages.