CAJ | 학술논문

目的探讨金雀异黄素、大豆黄素2种主要的植物雌激素对5/6肾切除鼠残余肾组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)沉积的影响及其机制.方法 32只鼠作5/6肾切除,将术后2周存活的27只鼠分为金雀异黄素组(9只)、大豆黄素组(10只)和对照组(8只),另有8只为假手术组.检测各组术前、术后第4、8周生化指标.观察第8 周肾组织的病理改变情况,应用免疫组化法检测肾组织中α-SMA的沉积情况,采用RT-PCR法和Western Blot法检测肾组织中转化生长因子-β1(TGF-β1)mRNA转录以及蛋白质表达情况.结果与对照组相比,金雀异黄素组和大豆黄素组尿蛋白排泄量明显减少,血肌酐、尿素氮水平下降(P<0.01),肾小球硬化程度明显减轻,肾小球内α-SMA沉积显著减少,TGF-β1 mRNA及其蛋白质表达明显减弱(P<0.05).结论金雀异黄素和大豆黄素可以降低5/6肾切除鼠残肾组织α-SMA的沉积,原因可能与抑制TGF-β1的表达有关.
목적 탐토금작이황소、대두황소2충주요적식물자격소대5/6신절제서잔여신조직α-평활기기동단백(α-smooth muscle actin,α-SMA)침적적영향급기궤제.방법 32지서작5/6신절제,장술후2주존활적27지서분위금작이황소조(9지)、대두황소조(10지)화대조조(8지),령유8지위가수술조.검측각조술전、술후제4、8주생화지표.관찰제8 주신조직적병리개변정황,응용면역조화법검측신조직중α-SMA적침적정황,채용RT-PCR법화Western Blot법검측신조직중전화생장인자-β1(TGF-β1)mRNA전록이급단백질표체정황.결과 여대조조상비,금작이황소조화대두황소조뇨단백배설량명현감소,혈기항、뇨소담수평하강(P<0.01),신소구경화정도명현감경,신소구내α-SMA침적현저감소,TGF-β1 mRNA급기단백질표체명현감약(P<0.05).결론 금작이황소화대두황소가이강저5/6신절제서잔신조직α-SMA적침적,원인가능여억제TGF-β1적표체유관.
Objective To study the effect of genistein and daidzein, the 2 main phytoestrogens, on the deposition of α-smooth muscle actin (α-SMA) in 5/6 nephrectomized rats and its related mechanism. Methods The 5/6 nephrectomized rats were randomly divided into the genistein group (n=9), the daidzein group (n=10), the control group (n=8), and still another 8 rats with sham-operation were used as control. Urinary protein, serum albumin, total protein, creatinine, urea were measured both before operation and 4 and 8 weeks after operation. Pathologic changes in the kidney were investigated. Immunohistochemistry was employed to investigate the expression of α-SMA. RT-PCR and Western Blot were also used to measure mRNA transcription and protein expression of transforming growth factor-β1 ( TGF-β1 ) respectively. Results Urinary albumin excretion, serum creatinine and urea in the genistein and daidzein groups decreased substantially when compared with those of the control group (P<0.01). Pathological lesion of the genistein group was less serious than that of the control group. The deposition of α-SMA was lower than that of the control group. Protein expression and mRNA transcription of TGF-β1 in the operated groups were attenuated by genistein and daidzein (P<0.05). Conclusions Genistein and daidzein could decrease the deposition of α-SMA, and this might be associated with the inhibition of the expression of TGF-β1.