CAJ | 학술논문

目的观察高能冲击波对肾脏钙离子腺苷三磷酸酶(Ca2+-ATPase)活性的影响及川芎嗪的保护作用,探讨高能冲击波肾损伤机制.方法 30只健康家兔制成单肾动物模型,按完全随机设计法分为对照组10只、高能冲击波组10只和川芎嗪组10只,对照组、高能冲击波组分别静脉注射等量生理盐水,高能冲击波冲击肾脏前3天,川芎嗪组静脉注射川芎嗪;冲击肾脏24h后,光学法检测肾细胞膜和线粒体膜Ca2+-ATPase活性;采用原位缺口末端标记法和流式细胞术检测凋亡细胞;采用生化分析测定内生肌酐清除率.结果与对照组比较,高能冲击波组肾细胞膜和线粒体膜Ca2+-ATPase活性均降低(P<0.05,P<0.01)、细胞凋亡率增加(P<0.01)、内生肌酐清除率降低(P<0.01);川芎嗪组肾细胞膜Ca2+-ATPase活性和内生肌酐清除率变化不明显(P＞0.05),线粒体膜Ca2+-ATPase活性降低(P<0.05)、细胞凋亡率增加(P<0.05),但幅度明显小于高能冲击波组.结论高能冲击波冲击肾脏后肾脏Ca2+-ATPase活性降低是发生肾细胞凋亡导致肾功能损伤的重要机制,川芎嗪有改善肾功能的作用,与其阻制肾脏Ca2+-ATPase活性降低、抗细胞凋亡有关.
목적 관찰고능충격파대신장개리자선감삼린산매(Ca2+-ATPase)활성적영향급천궁진적보호작용,탐토고능충격파신손상궤제.방법 30지건강가토제성단신동물모형,안완전수궤설계법분위대조조10지、고능충격파조10지화천궁진조10지,대조조、고능충격파조분별정맥주사등량생리염수,고능충격파충격신장전3천,천궁진조정맥주사천궁진;충격신장24h후,광학법검측신세포막화선립체막Ca2+-ATPase활성;채용원위결구말단표기법화류식세포술검측조망세포;채용생화분석측정내생기항청제솔.결과 여대조조비교,고능충격파조신세포막화선립체막Ca2+-ATPase활성균강저(P<0.05,P<0.01)、세포조망솔증가(P<0.01)、내생기항청제솔강저(P<0.01);천궁진조신세포막Ca2+-ATPase활성화내생기항청제솔변화불명현(P＞0.05),선립체막Ca2+-ATPase활성강저(P<0.05)、세포조망솔증가(P<0.05),단폭도명현소우고능충격파조.결론 고능충격파충격신장후신장Ca2+-ATPase활성강저시발생신세포조망도치신공능손상적중요궤제,천궁진유개선신공능적작용,여기조제신장Ca2+-ATPase활성강저、항세포조망유관.
Objective To observe the effects of high energy shock wave on Ca2+-ATPase activity in rabbit renal tissues with and protective effects of ligustrazine. Methods Thirty healthy rabbits were made into the mono-nephron models and then divided into 3 groups: control group, HESW group and HESW plus ligustrazine pretreated group. Rabbit kidney tissues were analyzed 24 hours after HESW. The activity of Ca2+-ATPase in cell membranes and mitochondriat fractions in renal tissue were analyzed with optical method. Cell apoptosis was detected by TdT-mediated dUTP Nick End Labelling (TUNEL) and flow cytometry. The variations of creatinine clearance (CCr) were observed.Results Compared with the control group, The activity of Ca2+-ATPase in cell membranes and mitochondrial fractions reduced, the rate of apoptosis increased and CCr reduced significantly in the HESW group. The activity of Ca2+-ATPase in cell membranes and CCr showed no significant difference between the HESW and ligustrazine pretreated group and the control group. The activity of Ca2+-ATPase reduced in mitochondrial fractions (P＜0.05)and cell apoptosis rate were significantly higher (P＜0.05) in the HESW plus ligustrazine pretreated group than the control group, but showed the highest level in the HESW group. Conclusion Ca2+-ATPase reduction and cell apoptosis increases are important mechanisms in the kidney function damage caused by HEWS. Lignstrazine can improve kidney function by suppressing kidney Ca2+-ATPase activity reduce and cell apoptosis.