中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
1期
38-40
,共3页
高婷婷%宋继权%Ahmad T.Khalaf%余想萍%程艳丽%高虎
高婷婷%宋繼權%Ahmad T.Khalaf%餘想萍%程豔麗%高虎
고정정%송계권%Ahmad T.Khalaf%여상평%정염려%고호
目的 探讨湖北汉族人群OX40配体蛋白基因rs844648位点和rs3850641位点基因多态性与SLE的相关性.方法 SLE患者82例和正常人对照组100例,采用PCR及限制性片段长度多态性方法(PCR-RFLP)检测TNFSF4基因rs844648和rs3850641位点多态性分布.结果 ①SLE组rs844648位点AA、AG和GG基因型频率分别为20.7％、62.2％、17.1％,正常人对照组为14.0％、55.0％、31.0％.SLE组rs844648多态性位点A等位基因携带者显著高于正常人对照组[73.2％比69.0％％,x2=4.69,P＜0.05,OR值=2.182( 1.068～ 4.458)],SLE组与正常人对照组间差异有统计学意义；②SNP位点rs3850641的正常人对照组AA、AG和GG基因型频率分别是76.0％、21.0％和3.0％,而SLE组分别为62.2％、31.7％和6.1％,SLE组rs3850641位点G等位基因携带者显著高于正常人对照组[37.8％比24.0％,x2=4.07,P＜0.05,OR值=1.925 (1.015 ～ 3.651)],SLE组与正常人对照组间差异有统计学意义.结论 TNFSF4基因rs844648和rs3850641位点存在单核苷酸多态性变异,该多态性与湖北地区汉族人群SLE的发病有相关性.
目的 探討湖北漢族人群OX40配體蛋白基因rs844648位點和rs3850641位點基因多態性與SLE的相關性.方法 SLE患者82例和正常人對照組100例,採用PCR及限製性片段長度多態性方法(PCR-RFLP)檢測TNFSF4基因rs844648和rs3850641位點多態性分佈.結果 ①SLE組rs844648位點AA、AG和GG基因型頻率分彆為20.7％、62.2％、17.1％,正常人對照組為14.0％、55.0％、31.0％.SLE組rs844648多態性位點A等位基因攜帶者顯著高于正常人對照組[73.2％比69.0％％,x2=4.69,P＜0.05,OR值=2.182( 1.068～ 4.458)],SLE組與正常人對照組間差異有統計學意義；②SNP位點rs3850641的正常人對照組AA、AG和GG基因型頻率分彆是76.0％、21.0％和3.0％,而SLE組分彆為62.2％、31.7％和6.1％,SLE組rs3850641位點G等位基因攜帶者顯著高于正常人對照組[37.8％比24.0％,x2=4.07,P＜0.05,OR值=1.925 (1.015 ～ 3.651)],SLE組與正常人對照組間差異有統計學意義.結論 TNFSF4基因rs844648和rs3850641位點存在單覈苷痠多態性變異,該多態性與湖北地區漢族人群SLE的髮病有相關性.
목적 탐토호북한족인군OX40배체단백기인rs844648위점화rs3850641위점기인다태성여SLE적상관성.방법 SLE환자82례화정상인대조조100례,채용PCR급한제성편단장도다태성방법(PCR-RFLP)검측TNFSF4기인rs844648화rs3850641위점다태성분포.결과 ①SLE조rs844648위점AA、AG화GG기인형빈솔분별위20.7％、62.2％、17.1％,정상인대조조위14.0％、55.0％、31.0％.SLE조rs844648다태성위점A등위기인휴대자현저고우정상인대조조[73.2％비69.0％％,x2=4.69,P＜0.05,OR치=2.182( 1.068～ 4.458)],SLE조여정상인대조조간차이유통계학의의；②SNP위점rs3850641적정상인대조조AA、AG화GG기인형빈솔분별시76.0％、21.0％화3.0％,이SLE조분별위62.2％、31.7％화6.1％,SLE조rs3850641위점G등위기인휴대자현저고우정상인대조조[37.8％비24.0％,x2=4.07,P＜0.05,OR치=1.925 (1.015 ～ 3.651)],SLE조여정상인대조조간차이유통계학의의.결론 TNFSF4기인rs844648화rs3850641위점존재단핵감산다태성변이,해다태성여호북지구한족인군SLE적발병유상관성.
Objective To investigate the association of two single nucleotide polymorphisms (SNPs),rs844648 and rs3850641,in OX40L (TNFSF4) gene,with systemic lupus erythematosus(SLE) in Hubei Han populations.Methods A total of 82 patients with SLE and 100 normal human controls were eligible for this study.Blood samples were obtained from these subjects and DNA was extracted from these samples.PCR-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the genotype of SNPs rs844648 and rs3850641.Results The frequency of AA,AG and GG genotype of the SNP rs844648 was 14.0％,55.0％and 31.0％ respectively in the controls,20.7％,62.2％ and 17.1％ respectively in the patients.A higher frequency was observed for A allele at the SNP rs844648 and G allele at the SNP rs3850641 in patients compared with the normal controls [73.2％ vs.69.0％％,x2 =4.69,P ＜ 0.05,OR =2.182 (1.068 - 4.458);37.8％ vs.24.0％,x2 =4.07,P ＜ 0.05,OR =1.925 (1.015 - 3.651)].The frequency of AA,AG and GG genotype of SNP rs3850641 was 76.0％,21.0％ and 3.0％ respectively in the normal controls,62.2％,31.7％ and 6.1％ respectively in the patients.Conclusions There are polymorphisms in the SNPs rs844648 and rs3850641 in TNFSF4 gene,which may be associated with the development of SLE.
