CAJ | 학술논문

患者女,16岁,左耳廓、面颊斑块3年.皮损取材真菌培养:沙氏葡萄糖琼脂培养基上菌落呈灰黑或灰褐色短绒毛状;马铃薯葡萄糖琼脂培养基小培养见分生孢子梗不分支或偶有分支,多芽合轴式产孢.分生孢子不分隔或有1～2个横隔,顶端钝圆,基底呈截断样;最适生长温度26℃～30℃,能液化明胶,水解淀粉.体外药敏实验对伊曲康唑、特比萘芬和两性霉素B敏感.对氟康唑耐药.组织病理检查见棕色菌丝和芽生孢子.PCR扩增ITS1-ITS4.片段并测序,与基因库中葡萄孢佛隆那霉标准株序列比对,结果99%同源.诊断:葡萄孢佛隆那霉所致的皮肤暗色丝孢霉病.治疗:口服伊曲康唑胶囊100 mg每日2次,疗程约8个月,皮损逐渐消退.
환자녀,16세,좌이곽、면협반괴3년.피손취재진균배양:사씨포도당경지배양기상균락정회흑혹회갈색단융모상;마령서포도당경지배양기소배양견분생포자경불분지혹우유분지,다아합축식산포.분생포자불분격혹유1～2개횡격,정단둔원,기저정절단양;최괄생장온도26℃～30℃,능액화명효,수해정분.체외약민실험대이곡강서、특비내분화량성매소B민감.대불강서내약.조직병리검사견종색균사화아생포자.PCR확증ITS1-ITS4.편단병측서,여기인고중포도포불륭나매표준주서렬비대,결과99%동원.진단:포도포불륭나매소치적피부암색사포매병.치료:구복이곡강서효낭100 mg매일2차,료정약8개월,피손축점소퇴.
A 16-year-old woman presented plaques on the left auricle and face over a period of 3 years. Fungal culture grew black-grey or dust velvety colony on Sabouraud's dextrose agar plate. A slide culture on potato dextrose agar plate showed conidiophores which were unbranched or occasionally loosely branched. The conidia were sympodial, zero- to two- septate, with rounded apices and truncated bases. The optimum growth temperature was 26℃ - 30℃. The fungus had the ability to liquefy glutin and hydrolyze starch. Anti-fungal susceptibility test showed the fungus was susceptible to itraconazole, terbinafine and amphoterecin B, but resistant to fluconazole. Cutaneous biopsy specimens revealed brown hyphae and budding yeast cells. The sequence of internal transcribed spacer (ITS) 1-ITS4 region of the isolate rDNA was assessed and compared against the Genebank databases. A 99% consistence was observed in the ITS sequence between clinical isolate and reference strain of Veronaea botryose Ciferri et Momtemartini. Based on the above findings, the mold was identified as Veronaea botryose Ciferri et Momtemartini. The lesions gradually subsided after 8-month treatment with oral itraconazole of 100 mg twice daily.