中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
2期
102-105
,共4页
肝炎病毒,甲型%基因型%序列分析
肝炎病毒,甲型%基因型%序列分析
간염병독,갑형%기인형%서렬분석
Hepatitis A virus%Genotype%Sequence analysis
目的 分析中国部分甲肝病毒流行株结构蛋白VP3-VP1区基因特点.方法 收集42份甲肝患者急性期血清标本,经核酸提取、逆转录及巢氏PCR,测得结构-非结构蛋白VP3-VP1-2A区序列,进行序列同源性比较并分析其基因特点.结果 42株HAV病毒株在VP1-2A连接处核苷酸和氨基酸序列同源性分别为89.1%～100%和97.3%～100%;在全长结构蛋白VP3-VP1区的核苷酸和氨基酸序列同源性分别为87.6%～100%和98.8%～100%.VP1-2A连接处序列相同的病毒株在全长结构蛋白VP3-VP1区的核苷酸同源性为98.4%～100%,0～2个氨基酸位点不同.本实验所得序列在中和抗原位点处氨基酸序列均未变异.结论 42株病毒株均属于I型,40株是IA亚型,2株IB亚型.本实验所用HAV流行株在结构蛋白VP3-VP1区的核苷酸存在差异但是氨基酸序列高度保守且没有中和抗原位点处的变异.VP1-2A结合处核苷酸序列相同的分离株在全长结构蛋白VP3-VP1区核苷酸序列相同或相近,氨基酸序列保守.
目的 分析中國部分甲肝病毒流行株結構蛋白VP3-VP1區基因特點.方法 收集42份甲肝患者急性期血清標本,經覈痠提取、逆轉錄及巢氏PCR,測得結構-非結構蛋白VP3-VP1-2A區序列,進行序列同源性比較併分析其基因特點.結果 42株HAV病毒株在VP1-2A連接處覈苷痠和氨基痠序列同源性分彆為89.1%～100%和97.3%～100%;在全長結構蛋白VP3-VP1區的覈苷痠和氨基痠序列同源性分彆為87.6%～100%和98.8%～100%.VP1-2A連接處序列相同的病毒株在全長結構蛋白VP3-VP1區的覈苷痠同源性為98.4%～100%,0～2箇氨基痠位點不同.本實驗所得序列在中和抗原位點處氨基痠序列均未變異.結論 42株病毒株均屬于I型,40株是IA亞型,2株IB亞型.本實驗所用HAV流行株在結構蛋白VP3-VP1區的覈苷痠存在差異但是氨基痠序列高度保守且沒有中和抗原位點處的變異.VP1-2A結閤處覈苷痠序列相同的分離株在全長結構蛋白VP3-VP1區覈苷痠序列相同或相近,氨基痠序列保守.
목적 분석중국부분갑간병독류행주결구단백VP3-VP1구기인특점.방법 수집42빈갑간환자급성기혈청표본,경핵산제취、역전록급소씨PCR,측득결구-비결구단백VP3-VP1-2A구서렬,진행서렬동원성비교병분석기기인특점.결과 42주HAV병독주재VP1-2A련접처핵감산화안기산서렬동원성분별위89.1%～100%화97.3%～100%;재전장결구단백VP3-VP1구적핵감산화안기산서렬동원성분별위87.6%～100%화98.8%～100%.VP1-2A련접처서렬상동적병독주재전장결구단백VP3-VP1구적핵감산동원성위98.4%～100%,0～2개안기산위점불동.본실험소득서렬재중화항원위점처안기산서렬균미변이.결론 42주병독주균속우I형,40주시IA아형,2주IB아형.본실험소용HAV류행주재결구단백VP3-VP1구적핵감산존재차이단시안기산서렬고도보수차몰유중화항원위점처적변이.VP1-2A결합처핵감산서렬상동적분리주재전장결구단백VP3-VP1구핵감산서렬상동혹상근,안기산서렬보수.
Objective To analyse the genetic characteristics of the capsid protein VP3-VP1 region of hepatitis A virus strains circulated in China. Methods The nucleotide sequences of VP3-VP1-2A region of 42 HAV IgM positive serum samples were sequenced and analysed for nucleotide and amino acid identities and genetic characteristics of the VP3-VP1 region. Results The nucleotide and amino acid identities in the VP1-2A junction region among the 42 strains were 89. 1% -100% and 97.3% -100% ; while in the complete VP3-VP1 region, the identities were 87.6%-100% and 98.8%-100%. Strains with identical nucleotide sequences in the VP1-2A junction region had 98.4%-100% nucleotide identity in the complete VP3-VP1 region and 0-2 amino acid differences in this region. There were no amino acid changes at neutralizing antigenetic sites of VP3-VP1 region within the 42 HAV strains. Conclusion All the 42 HAV strains belonged to genotype I, with 40 strains clustering to sub-genotype IA and 2 to sub-genotype IB. Different HAV strains analysed in this paper differed in the nueleotide sequences of the VP3-VP1 region, but the amino acid sequences were highly conserved with no changes at neutralizing antigenetic sites. Both the nucleotide and amino acid sequences of the strains with the same VP1-2A junction region were identical or closely related when compared in the complete VP3-VP1 region.
