CAJ | 학술논문

目的观察锌指转录因子Snail及上皮钙黏蛋白(E-cadherin)在肾透明细胞癌(CCRCC)中的表达,并探讨与CCRCC病理分级、分期和转移等相关生物学行为因素的关系.方法收集我院手术切除的69例CCRCC组织标本,同时取58例癌旁组织和10例正常肾组织标本作为对照,采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结(SP)法检测CCRCC组织、癌旁组织和正常肾组织中Snail和E-cadherin的表达.结果 CCRCC组织Snail阳性率(82.7％)显著高于癌旁组织(43.1％,P＜0.01).CCRCC组织E-cadherin阳性率(31.9％)显著低于癌旁组织(91.5％)(P＜0.01).两者的表达均与CCRCC不同分化程度、分期、浸润和转移有关(P＜0.05).CCRCC组织中E-cadherin与Snail的表达呈负相关(r=-0.342,P＜0.05).结论 E-cadherin低表达与Snail 高表达可能是CCRCC发生浸润转移的重要生物学标志.联合检测E-cadherin及Snail对预测CCRCC浸润转移有重要意义.
목적 관찰자지전록인자Snail급상피개점단백(E-cadherin)재신투명세포암(CCRCC)중적표체,병탐토여CCRCC병리분급、분기화전이등상관생물학행위인소적관계.방법 수집아원수술절제적69례CCRCC조직표본,동시취58례암방조직화10례정상신조직표본작위대조,채용면역조직화학련매균항생물소단백-과양화물매련결(SP)법검측CCRCC조직、암방조직화정상신조직중Snail화E-cadherin적표체.결과 CCRCC조직Snail양성솔(82.7％)현저고우암방조직(43.1％,P＜0.01).CCRCC조직E-cadherin양성솔(31.9％)현저저우암방조직(91.5％)(P＜0.01).량자적표체균여CCRCC불동분화정도、분기、침윤화전이유관(P＜0.05).CCRCC조직중E-cadherin여Snail적표체정부상관(r=-0.342,P＜0.05).결론 E-cadherin저표체여Snail 고표체가능시CCRCC발생침윤전이적중요생물학표지.연합검측E-cadherin급Snail대예측CCRCC침윤전이유중요의의.
Objective To study the expression of transcriptional factor Snail and adhesion factor E-cadherin in renal clear cell carcinoma (RCCC) and evaluate their correlation with pathological grading,clinical stage,invasion and metastases of RCCC.Methods The expression of Snail and E-cadherin proteins was detected by using immunohistochemical streptavidin-peroxidase method in 69 cases of RCCC tissue,58 cases of the para-cancerous mucosa and 10 cases of normal renal tissue.Results The positive rate of Snail in RCCC was 82.7％,which was significantly higher than that in para-cancerous mucosa (43.1％,P ＜0.01 ).The positive rate of E-cadherin in RCCC was 31.9％,which was significantly lower than that in para-cancerous mucosa (91.5％,P ＜0.01 ).The expression of E-cadherin and Snail was significantly related with the differential degrees,clinical stage,depth of tumor invasion and distant metastasis ( P ＜ 0.05 ).There was a negative correlation between the expression of E-cadherin and Snail in RCCC.Conclusion The over-expression of Snail and decreased expression of E-cadherin might be important biological markers for invasion and metastasis of RCCC.The combined detection of E-cadherin and Snail has fas-reaching significance on predicting the invasion and metastasis of RCCC.