CAJ | 학술논문

目的探讨趋化因子基质细胞衍生因子-1(SDF-1)及趋化因子受体4(CXCR4)对胃癌腹膜转移潜能的影响.方法采用逆转录多聚酶链式反应(RT-PCR)检测胃癌细胞NUGC4及间皮细胞HMrSV中CXCR4和SDF-1 mRNA的表达.采用MTT实验检测NUGC4细胞增殖能力的变化,采用间皮细胞黏附实验及间皮细胞迁移实验评价体外培养的NUGC4细胞与间皮细胞发生黏附的能力及其穿越间皮细胞发生迁移的能力.通过建立裸鼠胃癌腹膜种植瘤模型,评价NUGCA细胞腹膜肿瘤生成能力及荷瘤裸鼠生存时间的变化.结果 NUGC4细胞表达较高强度的CXCR4mRNA,而间皮细胞表达高强度的SDF-1 mRNA.抗CXCR4单抗对NUGC4细胞增殖具有显著的抑制作用(P＜0.05);SDF-1可促进NUGC4细胞与间皮细胞黏附及穿越间皮细胞发生迁移,抗CXCR4单抗处理可显著抑制NUGC4细胞的黏附及迁移能力(P＜0.05).体内实验结果显示,CXCR4拮抗剂AMD3100治疗组裸鼠的平均生存时间显著长于对照组[(43.8±2.8)vs(28.2±2.5)d,P＜0.01],瘤结节数目显著低于对照组[(64.6±8.2)vs(103±12.4),P＜0.01].结论趋化因子SDF-1及其受体CXCR4参与胃癌腹膜转移过程,与肿瘤细胞增殖、肿瘤细胞-问皮细胞问的黏附和肿瘤细胞迁移等步骤密切相关;干扰SDF-1/CXCR4生物学轴可以作为胃癌腹膜转移的潜在治疗策略.
목적 탐토추화인자기질세포연생인자-1(SDF-1)급추화인자수체4(CXCR4)대위암복막전이잠능적영향.방법 채용역전록다취매련식반응(RT-PCR)검측위암세포NUGC4급간피세포HMrSV중CXCR4화SDF-1 mRNA적표체.채용MTT실험검측NUGC4세포증식능력적변화,채용간피세포점부실험급간피세포천이실험평개체외배양적NUGC4세포여간피세포발생점부적능력급기천월간피세포발생천이적능력.통과건립라서위암복막충식류모형,평개NUGCA세포복막종류생성능력급하류라서생존시간적변화.결과 NUGC4세포표체교고강도적CXCR4mRNA,이간피세포표체고강도적SDF-1 mRNA.항CXCR4단항대NUGC4세포증식구유현저적억제작용(P＜0.05);SDF-1가촉진NUGC4세포여간피세포점부급천월간피세포발생천이,항CXCR4단항처리가현저억제NUGC4세포적점부급천이능력(P＜0.05).체내실험결과현시,CXCR4길항제AMD3100치료조라서적평균생존시간현저장우대조조[(43.8±2.8)vs(28.2±2.5)d,P＜0.01],류결절수목현저저우대조조[(64.6±8.2)vs(103±12.4),P＜0.01].결론 추화인자SDF-1급기수체CXCR4삼여위암복막전이과정,여종류세포증식、종류세포-문피세포문적점부화종류세포천이등보취밀절상관;간우SDF-1/CXCR4생물학축가이작위위암복막전이적잠재치료책략.
Objective To investigate the effect of chemokine stromal cell-derived factor-1(SDF-1) and its receptor CXCR4 on the peritoneal earcinometastasis of gastric cancer.Methods Human gastric cancer cells of tlle lie NUGC4 and mesothelial cells of the line HMrSV were cultured.RT-PCR was used to detect the expression of CXCR4 and SDF-1 mRNA in the NUGCA and HMrSV cells.The proliferation of NUGCA cells was detected bv MTT method.In mesothelial cell adhesion teat NUGC4 cells were cultured with confluent HMrSV mesothelial cells in 24-well plate and then divided into 3 groups:chemokine group,added with SDF-1,antibody blocking group,in which the NUGCA cells were pre-incubated with CXCR4 monoclonal antibody for 2 h and then SDF-1 was added,and control group added with only culture fluid.Microscopy was used to calculate the number of gastric cancer cells adhered with mesothelial cells.In the mesothelial cell migration test HMrSV cells were put in the upper chamber of a Transwell chamber so as to cover tlle infiltration membrane.This Transwell chamber was put into a culture plate,NUGC4 cells,divided into 3 groups as mentioned above were put into the upper chamber,24 h later HE staining and microscopy were performed to calculate the number of the NUGC4 cells that penetrated the membrane.BALB/c nu/nu female nude mice underwent intraperitoneal injection of NUGC4 cells,and then with PBS or AMD3100,small molecular specific antagonist,one day after the cancer cell injection once a day for 2 weeks.Then the mice were killed to observe the intraperitoneal tumorogenesis.Results CXCR4 mRNA was highly expressed in the NUGCA cells but only very weekly expressed in the HMrSV cells.SDF-1 mRNA expression was seen in the HMrSV cells but in the NUGC4 cells.Anti-CXCR4 monoclonal antibody(Mcab)inhibited the proliferation of NUGC4 cells significantly(P＜0.05).In mesothelial cell adhesion test,the number of the NUGC4 cells adhered with HMrSV cells after SDF-1 stimulation was 84.4±21.2,significantly higher than that of the control group(43.6±12.4,P＜0.05).The number of migrating NUGC4 cells in the chemokine group was 170.8±24.2,significantly higher than hat of the control group(102.8±18.2,P＜0.05);and the number of migrating NUGC4 cells in the antibody blocking group was 114.7±20.3,significantly lower than that of the chemokine group(P＜0.05).The survival time of the mice injected with both NUGC4 cells and AMD3100 was(43.8±2.8)days,significantly longer than that of the control group[(28.2±2.5) days,P＜0.01].The tumor number of the AMD3100 group was(54.6±8.2),significantly lower than that of the control group[(103±12.4),P＜0.01].Conclusion SDF-1 and its receptor CXCR4 play an important role in the development of peritoneal carcinometastasis from gastric cancer. Interfering with the SDF-1/CXCR4 biological axis may become a potential strategy in the prevention and treatment of peritoneal carcinometastasis from gastric cancer.