CAJ | 학술논문

实验采用细胞外玻璃微电极采集豚鼠海马神经元放电信号,并将信号转化为峰峰间期(interspike interval,ISI)以研究麻醉和清醒状态海马锥体细胞自发放电线性和非线性特点.实验建立了豚鼠海马锥体细胞与中间神经元电生理鉴别标准;麻醉和清醒状态下豚鼠海马CAl和CA3区锥体细胞自发放电频率、时程、复杂度等无显著区别;麻醉组豚鼠海马锥体细胞ISI序列的复杂度小于清醒组,锥体细胞分型和ISI变异度等表现不同.实验表明,麻醉和清醒状态下豚鼠海马锥体细胞自发放电呈不同线性和非线性特征.传统和非线性研究手段的结合,可能较全面地反映海马锥体细胞自发放电特性.
실험채용세포외파리미전겁채집돈서해마신경원방전신호,병장신호전화위봉봉간기(interspike interval,ISI)이연구마취화청성상태해마추체세포자발방전선성화비선성특점.실험건립료돈서해마추체세포여중간신경원전생리감별표준;마취화청성상태하돈서해마CAl화CA3구추체세포자발방전빈솔、시정、복잡도등무현저구별;마취조돈서해마추체세포ISI서렬적복잡도소우청성조,추체세포분형화ISI변이도등표현불동.실험표명,마취화청성상태하돈서해마추체세포자발방전정불동선성화비선성특정.전통화비선성연구수단적결합,가능교전면지반영해마추체세포자발방전특성.
To determine the characteristics of spontaneous discharges of hippocampal pyramidal cells (PCs), extracellular neuronal firing in CA1 and CA3 regions of dorsal hippocampus was recorded, the firing modes and interspike interval (ISI) were analyzed with the conventional and nonlinear methods. PCs were discriminated from interneurons using the measurement of action-potential duration and firing rate in this study. There was no significant difference in duration, mean firing frequency, complexity and firing mode between the neurons in CA1 and CA3 regions both in anesthetized and awake animals. The complexity of neurons was higher in awake group than that in anesthetized group, though no difference was found in firing rate. There were differences in the type of pyramidal cells and the coefficient of variance of ISI of neurons. The results obtained from the spontaneous discharges of dorsal hippocampal pyramidal cells reveal some nonlinear and linear aspects in anesthetized and awake states. It seems likely that the combination of conventional and non-linear measurements of the hippocampal pyramidal cells encoding may reflect genuine characteristics of the hippocampal pyramidal cells.