CAJ | 학술논문

目的观察右旋松萝酸的口服急性毒性,MTT方法测其细胞抑制活性.方法建立小鼠经口急性中毒小鼠模型,观察不同剂量药物灌胃小鼠的中毒表现.测定小鼠的经口LD_(50),观察不同剂量右旋松萝酸对小鼠生存时间及存活率的影响.同时,用不同浓度的右旋松萝酸作用于体外培养的心肌成纤维细胞,MTT方法测定细胞的IC_(50),观察不同浓度药物对细胞的抑制率.结果右旋松萝酸剂量越大,小鼠的中毒症状越明显,生存时间越短.小鼠经口LD-(50)为388mg/kg,中毒表现主要有精神萎靡,耸毛,畏寒,呼吸急促,行动迟缓,厌食等症状.体外细胞培养实验显示,右旋松萝酸对细胞增值的抑制呈剂量正相关,较大剂量对细胞有破坏作用,其对心肌成纤维细胞的IC_(50)为322 μg/ml.结论右旋松萝酸为一种中低毒的天然化合物,中低剂量没有明显的细胞毒性.
목적 관찰우선송라산적구복급성독성,MTT방법측기세포억제활성.방법 건립소서경구급성중독소서모형,관찰불동제량약물관위소서적중독표현.측정소서적경구LD_(50),관찰불동제량우선송라산대소서생존시간급존활솔적영향.동시,용불동농도적우선송라산작용우체외배양적심기성섬유세포,MTT방법측정세포적IC_(50),관찰불동농도약물대세포적억제솔.결과 우선송라산제량월대,소서적중독증상월명현,생존시간월단.소서경구LD-(50)위388mg/kg,중독표현주요유정신위미,용모,외한,호흡급촉,행동지완,염식등증상.체외세포배양실험현시,우선송라산대세포증치적억제정제량정상관,교대제량대세포유파배작용,기대심기성섬유세포적IC_(50)위322 μg/ml.결론 우선송라산위일충중저독적천연화합물,중저제량몰유명현적세포독성.
Objective To observe the oral acute toxicity of of (+)-usnic acid in mice and assess its cytotoxicity in rat cardiac fibroblasts. Methods The mice with acute poisoning of (+)-usnic acid at different doses by oral administration were observed for toxic manifestations, and the LD_(50) was determined. The survival time and survival rate of the mice receiving different doses of (+)-usnic acid were observed. Cultured rat cardiac fibroblasts were inoculated with different concentrations of (+)-usnic acid, and the cell growth inhibition rate was estimated and the IC_(50), determined using MTT assay. Results Higher dose of (+) -usnic acid resulted in more obvious symptoms of poisoning and shorter survival time of the mice. The LD_(50) of (+)-usnic acid in mice by oral administration was 388 mg/kg. The manifestations of poisoning such as apathism, pilomotor, chill, dyspnea,torpidity and anorexia was observed. Rat cardiac fibroblasts incubated with (+)-usnic acid showed obvious growth inhibition,which was positively correlated to the dose of (+)-usnic acid, and high dose of (+)-usnic acid caused severe cell injuries. The IC_(50) of (+)-usnic acid in rat cardiac fibroblasts was 322 μg/ml. Conclusion (+)-usnic acid is a natural compound of low toxicity in mice, and low to medium dose of (+)-usnic acid dose not produce obvious cytotoxicity.