CAJ | 학술논문

目的:评价当前国内外微囊化人工细胞技术在糖尿病中的应用效果.方法:检索PUBMED数据库中1980/2009有关人工细胞微囊技术进展在糖尿病中应用的文献,检索词"microcapsule,islet,transplantation",并限定文献语言种类为English:同时检索中国期刊全文数据库1999/2009有关人工细胞微囊技术进展在糖尿病中应用的文献,检索词为"微囊,胰岛,移植".按纳入与排除标准收集所有相关动物随机对照及临床试验,评价其质量及提取数据.结果:共纳入25篇文献,包括46例1型糖尿病患者,106只实验鼠,20头实验猪,25只实验猴.结果显示,与对照组相比,未囊化组与微囊化组胰岛细胞移植均可分泌胰岛素和降低血糖,微囊胰岛细胞组的胰岛细胞作用和存活时间更长.微囊具有较好的免疫隔离作用.结论:微囊胰岛细胞移植后能改善和调节糖尿病的糖代谢异常,且微囊的免疫隔离作用能消除或减轻受体对异体或异种免疫排斥反应,减少或消除对免疫抑制剂的依赖.合适的囊材、合理的工艺、先进的制囊设备和胰岛分离提纯技术、适宜的移植位置能增强微囊胰岛细胞的抗机械强度,提高生物相容性,提高胰岛细胞移植的成功率,改善人工胰岛细胞的功能,延长存活时间.
목적:평개당전국내외미낭화인공세포기술재당뇨병중적응용효과.방법:검색PUBMED수거고중1980/2009유관인공세포미낭기술진전재당뇨병중응용적문헌,검색사"microcapsule,islet,transplantation",병한정문헌어언충류위English:동시검색중국기간전문수거고1999/2009유관인공세포미낭기술진전재당뇨병중응용적문헌,검색사위"미낭,이도,이식".안납입여배제표준수집소유상관동물수궤대조급림상시험,평개기질량급제취수거.결과:공납입25편문헌,포괄46례1형당뇨병환자,106지실험서,20두실험저,25지실험후.결과현시,여대조조상비,미낭화조여미낭화조이도세포이식균가분비이도소화강저혈당,미낭이도세포조적이도세포작용화존활시간경장.미낭구유교호적면역격리작용.결론:미낭이도세포이식후능개선화조절당뇨병적당대사이상,차미낭적면역격리작용능소제혹감경수체대이체혹이충면역배척반응,감소혹소제대면역억제제적의뢰.합괄적낭재、합리적공예、선진적제낭설비화이도분리제순기술、괄의적이식위치능증강미낭이도세포적항궤계강도,제고생물상용성,제고이도세포이식적성공솔,개선인공이도세포적공능,연장존활시간.
OBJECTIVE: To systematically evaluate the application of microcapsuled cell or encystation materials for treating diabetes mellitus at hone or abroad in recent years.METHODS: A computer-based online search of PubMed database was undertaken to identify related articles about the microcapsulated cell or encystation material for treating diabetes mellitus published from 1980 to 2009 by using the key words of "microcapsulation, islet, transplantation" in English. Meanwhile, we search CNKI for relevant articles published between 1999 and 2009 with the same key words in Chinese. The related randomized, controlled, and clinical studies were collected according to conclusion and exclusion criteria.RESULTS: Among 25 articles, there were 46 patients with type I diabetes mellitus, 106 rats, 20 pigs, and 25 monkeys. The experimental results showed that both the microcapsulated islets and non-microcapsulated islets could secrete insulin and decrease blood glucose level. The normal blood glucose level with microcapulated islets could be maintained for a long time.Thus, microcapsule had a great immune isolation reaction.CONCLUSION: After transplantation, microencapsulated islet cells could improve and adjust abnormal glycometabolism of patients with diabetes mellitus; furthermore, the immunoisolation effect of microcapsule could eliminate or relieve the immunological rejection of receptors to allograft or xenograft, while it could also relieve or eliminate dependence on immunosuppressive drugs. Appropriate material of microcapsule, reasonable process, advanced equipment, advanced separation and purification technologies of islet, and suitable site for transplantation could improve the function of artificial islet cell, enhance the anti-machinery and chemical strength of microencapsuiated islet, improve biocompatibility, and prolong survival time.