光谱实验室
光譜實驗室
광보실험실
CHINESE JOURNAL OF SPECTROSCOPY LABORATORY
2010年
2期
561-564
,共4页
液相色谱%电喷雾离子化%质谱%冬虫夏草%虫草素
液相色譜%電噴霧離子化%質譜%鼕蟲夏草%蟲草素
액상색보%전분무리자화%질보%동충하초%충초소
Liquid Chromatography%Electro-Spary Ionization Interface%Mass Spectrometry%Cord yceps Sinensis%Cordycepin
提出了一个灵敏度高、选择性好的测定冬虫夏草及其代用品中主要活性成分虫草亲含量的LC/ESI-MS方法.用甲醇为提取溶剂,采用选择性离子检测(sIM)和电喷雾离子化(ESI);以ShimadzuVP-ODS为色谱柱;流动相为水-甲醇-甲酸(87:12:1),2-氯腺苷为内标.虫草素回归方程为y=0.1947x+0.00161,r=0.9992,线性范围0.4-120.0μg·mL~(-1),虫草素平均回收率为98.2%.该方法已成功用于不同产地冬虫夏草及其代用品中虫草素含量的分析,可用于冬虫夏草及其代用品的质量控制.
提齣瞭一箇靈敏度高、選擇性好的測定鼕蟲夏草及其代用品中主要活性成分蟲草親含量的LC/ESI-MS方法.用甲醇為提取溶劑,採用選擇性離子檢測(sIM)和電噴霧離子化(ESI);以ShimadzuVP-ODS為色譜柱;流動相為水-甲醇-甲痠(87:12:1),2-氯腺苷為內標.蟲草素迴歸方程為y=0.1947x+0.00161,r=0.9992,線性範圍0.4-120.0μg·mL~(-1),蟲草素平均迴收率為98.2%.該方法已成功用于不同產地鼕蟲夏草及其代用品中蟲草素含量的分析,可用于鼕蟲夏草及其代用品的質量控製.
제출료일개령민도고、선택성호적측정동충하초급기대용품중주요활성성분충초친함량적LC/ESI-MS방법.용갑순위제취용제,채용선택성리자검측(sIM)화전분무리자화(ESI);이ShimadzuVP-ODS위색보주;류동상위수-갑순-갑산(87:12:1),2-록선감위내표.충초소회귀방정위y=0.1947x+0.00161,r=0.9992,선성범위0.4-120.0μg·mL~(-1),충초소평균회수솔위98.2%.해방법이성공용우불동산지동충하초급기대용품중충초소함량적분석,가용우동충하초급기대용품적질량공제.
A highly sensitive and selective method for the determination of cordycepin in cordyceps sinensis and its substitutes by LC/ESI-MS was developed.The electro-spray ionization (ESI) interface and selective ion monitoring (SIM) mode were used.The analytical column was 2.0 × 150mm shimadzu VP-ODS column and the mobile phase was water (87%), methanol (12%) and formic acid (1%).Methanol was used as extract solution and 2-chloroadenosine was used as internal standard.The regression equations is y=0.1947x+0.00161 with r=0.9992.The liner range the determination of cordycepin in cordyceps sinensis and its substitutes.This method also can be applied for the quality control of cordyceps sinensis and its substitutes.
