CAJ | 학술논문

目的分析不同亚型丙型肝炎病毒(HCV)包膜糖蛋白E1和E2的变异性.方法从HCV基因数据库中获得不同亚型HCV包膜序列,并利用生物信息学方法进行核苷酸序列变异性、包膜糖蛋白亲水性性及糖基化位点分析.结果与其他亚型相比,1b亚型HCV包膜糖蛋白E1和E2区核苷酸的变异性最高,且E1区的变异性高于E2区;在E1区55-72位和102-112位氨基酸处1b亚型亲水性较大;1b亚型的包膜糖蛋白E1区N-糖基化位点数最多,且在60位氨基酸处单独有一糖基化位点.结论与E2区相比,E1区的高突变可能与1b型HCV致病性较强关系密切,且1b亚型E1蛋白的大量糖基化也可能在病毒的致病及免疫逃逸中有一定作用.
목적 분석불동아형병형간염병독(HCV)포막당단백E1화E2적변이성.방법 종HCV기인수거고중획득불동아형HCV포막서렬,병이용생물신식학방법진행핵감산서렬변이성、포막당단백친수성성급당기화위점분석.결과 여기타아형상비,1b아형HCV포막당단백E1화E2구핵감산적변이성최고,차E1구적변이성고우E2구;재E1구55-72위화102-112위안기산처1b아형친수성교대;1b아형적포막당단백E1구N-당기화위점수최다,차재60위안기산처단독유일당기화위점.결론 여E2구상비,E1구적고돌변가능여1b형HCV치병성교강관계밀절,차1b아형E1단백적대량당기화야가능재병독적치병급면역도일중유일정작용.
Objective To analyze the variability of envelope glycoprotein (env) of hepatitis C virus.Methods HCV subtype 1a、1b,2a,2b and 3a nucleotide sequences, and amino-acid consensus sequence in the FASTA format were collected from the HCV Sequence Database (http://hcv. lanl. gov). Variation of the nucleotide sequence and the Hydrophilicity and glycosylation within envelope glycoprotein were analyzed. Results El and E2 region of HCV subtype 1b had the most variability, genetic variability of E1 was higher than that of E2 region. E1 region of HCV subtype 1b had higher hydrophilicity at position 55-72 and 102-112 of amino acid residues;E1 region of HCV subtype 1b had the most highest frequency glycosylation site and had a Nglycosylation site in position 60. Conclusions The high variability of El region and glycosylation may be associated with the pathogenicity of HCV subtype 1b.