中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2008年
9期
697-701
,共5页
受体,肾上腺素α1%受体,血管紧张素,2型
受體,腎上腺素α1%受體,血管緊張素,2型
수체,신상선소α1%수체,혈관긴장소,2형
Receptor adrenergie,alpha-1%Receptor,angiotensin,type 2
目的 从受体信使RNA(messenger RNA,mRNA)水平研究了心脏α1-肾上腺素受体(α1-AR)亚型和血管紧张素Ⅱ受体(ATR)亚型在大鼠增龄过程中表达水平的变化. 方法 采用半定量逆转录聚合酶链反应(semiquantitative reverse transcription polymerase chain reaction,RTPCR)分别对3月龄(青年)、12月龄(中年)、18月龄(老年前期)和24月龄(老年)Wistar大鼠左心室的mRNA水平进行测定. 结果 α1A-AR随增龄逐渐F调,各年龄组的表达水平分别为0.71±0.06、0.29±0.06、0.23±0.03、0.12±0.04.α1D-AR在中年和老年前期下凋,各年龄组的表达水平分别为0.52±0.05、0.41±0.03、0.24±0.11、0.27±0.08.α1B-AR的表达未随增龄而变化.AT1 R在老年组出现明显上调,各年龄组的表达水平分别为0.60±0.14、0.58±0.12、0.68±0.10、1.09±0.14.AT2R的表达水平在增龄过程中无明显变化. 结论 α1-AR哑型和ATR亚型的表达水平随年龄增长出现了明显改变,这可能是心脏在衰老过程中产生的适应性反应,并且对维持衰老心脏的生理功能具有重要意义.
目的 從受體信使RNA(messenger RNA,mRNA)水平研究瞭心髒α1-腎上腺素受體(α1-AR)亞型和血管緊張素Ⅱ受體(ATR)亞型在大鼠增齡過程中錶達水平的變化. 方法 採用半定量逆轉錄聚閤酶鏈反應(semiquantitative reverse transcription polymerase chain reaction,RTPCR)分彆對3月齡(青年)、12月齡(中年)、18月齡(老年前期)和24月齡(老年)Wistar大鼠左心室的mRNA水平進行測定. 結果 α1A-AR隨增齡逐漸F調,各年齡組的錶達水平分彆為0.71±0.06、0.29±0.06、0.23±0.03、0.12±0.04.α1D-AR在中年和老年前期下凋,各年齡組的錶達水平分彆為0.52±0.05、0.41±0.03、0.24±0.11、0.27±0.08.α1B-AR的錶達未隨增齡而變化.AT1 R在老年組齣現明顯上調,各年齡組的錶達水平分彆為0.60±0.14、0.58±0.12、0.68±0.10、1.09±0.14.AT2R的錶達水平在增齡過程中無明顯變化. 結論 α1-AR啞型和ATR亞型的錶達水平隨年齡增長齣現瞭明顯改變,這可能是心髒在衰老過程中產生的適應性反應,併且對維持衰老心髒的生理功能具有重要意義.
목적 종수체신사RNA(messenger RNA,mRNA)수평연구료심장α1-신상선소수체(α1-AR)아형화혈관긴장소Ⅱ수체(ATR)아형재대서증령과정중표체수평적변화. 방법 채용반정량역전록취합매련반응(semiquantitative reverse transcription polymerase chain reaction,RTPCR)분별대3월령(청년)、12월령(중년)、18월령(노년전기)화24월령(노년)Wistar대서좌심실적mRNA수평진행측정. 결과 α1A-AR수증령축점F조,각년령조적표체수평분별위0.71±0.06、0.29±0.06、0.23±0.03、0.12±0.04.α1D-AR재중년화노년전기하조,각년령조적표체수평분별위0.52±0.05、0.41±0.03、0.24±0.11、0.27±0.08.α1B-AR적표체미수증령이변화.AT1 R재노년조출현명현상조,각년령조적표체수평분별위0.60±0.14、0.58±0.12、0.68±0.10、1.09±0.14.AT2R적표체수평재증령과정중무명현변화. 결론 α1-AR아형화ATR아형적표체수평수년령증장출현료명현개변,저가능시심장재쇠로과정중산생적괄응성반응,병차대유지쇠로심장적생리공능구유중요의의.
Objective To examine the expression levels of α1-adrenergie receptor(α1-AR)and angiotensinⅡ reeeptor(ATR)subtypes in left ventricle of rats from adolescent age, middle age,presenium to senium. Methods Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was used to quantitate the messenger RNA (mRNA) of α1-AR and ATR subtypes in left ventricle in Wistar rats aged 3-months (adolescent age), 12-months (middle age), 18-months (presenium) and 24-months (senium). Results The expression of α1A-AR mRNA was decreased gradually with aging, and the gene expression of α1 D-AR was repressed in middle age and presenium,while the expression of a,B-AR mRNA remained unchanged during senescence. Cardiac AT1R expression was not affected by aging from adolescent age to presenium, but exhibited a remarkable up-regulation in senium There was no significant discrepancies of cardiac AT2R expression among the four different age groups. Conclusions The results suggest that there are considerable changes in mRNA levels of cardiac α1-AR and ATR subtypes with aging. The change of cardiac α1-AR and ATR expression during aging is a protective response for senescence and has an important significance in maintaining normal physiological functions of heart.
