中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
12期
1091-1093
,共3页
氟比洛芬%疼痛%手术后%镇痛
氟比洛芬%疼痛%手術後%鎮痛
불비락분%동통%수술후%진통
Flurbiprofen%Pain,postoperative%Analgesia
目的 评价氟比洛芬酯静脉注射后在大鼠手术切口部位肌肉组织中的分布.方法 清洁级雄性SD大鼠40只,体重240～260 g,随机分为5组(n=8):切口痛组(IP组)和不同剂量氟比洛芬酯组(F_(1～4)组).建立大鼠切口痛模型后20 min时IP组经尾静脉注射生理盐水0.5 ml,F_(1～4)组经尾静脉分别注射氟比洛芬酯2、4、8和16 mg/kg.于尾静脉注射结束后2 h时,采用反相高效液相色谱法测定肌肉氟比洛芬含量,采用ELISA法测定前列腺素E_2 (PGE_2)含量.结果 与非手术侧比较,F_(1～4)组手术侧足底肌肉组织氟比洛芬和PGE_2含量、IP组手术侧PGE_2含量升高(P<0.01);与IP组比较,F_(1～4)组手术侧足底肌肉组织PGE_2含量降低(P<0.05),F_4组非手术侧足底肌肉组织PGE_2含量降低,F_(1～3)组非手术侧上述指标差异无统计学意义(P>0.05);F_1组、F_2组、F_3组和F_4组手术侧足底肌肉组织氟比洛芬和PGE_2含量依次升高,F_4组非手术侧足底肌肉组织氟比洛芬和PGE_2含量高于F_1组、F_2组和F_3组(P<0.05或0.01),F_1组、F_2组和_3组非手术侧上述指标比较差异无统计学意义(P>0.05).结论 氟比洛芬酯静脉注射后在大鼠手术切口部位肌肉组织中的分布高于非手术侧,提示氟比洛芬酯具有靶向镇痛作用.
目的 評價氟比洛芬酯靜脈註射後在大鼠手術切口部位肌肉組織中的分佈.方法 清潔級雄性SD大鼠40隻,體重240～260 g,隨機分為5組(n=8):切口痛組(IP組)和不同劑量氟比洛芬酯組(F_(1～4)組).建立大鼠切口痛模型後20 min時IP組經尾靜脈註射生理鹽水0.5 ml,F_(1～4)組經尾靜脈分彆註射氟比洛芬酯2、4、8和16 mg/kg.于尾靜脈註射結束後2 h時,採用反相高效液相色譜法測定肌肉氟比洛芬含量,採用ELISA法測定前列腺素E_2 (PGE_2)含量.結果 與非手術側比較,F_(1～4)組手術側足底肌肉組織氟比洛芬和PGE_2含量、IP組手術側PGE_2含量升高(P<0.01);與IP組比較,F_(1～4)組手術側足底肌肉組織PGE_2含量降低(P<0.05),F_4組非手術側足底肌肉組織PGE_2含量降低,F_(1～3)組非手術側上述指標差異無統計學意義(P>0.05);F_1組、F_2組、F_3組和F_4組手術側足底肌肉組織氟比洛芬和PGE_2含量依次升高,F_4組非手術側足底肌肉組織氟比洛芬和PGE_2含量高于F_1組、F_2組和F_3組(P<0.05或0.01),F_1組、F_2組和_3組非手術側上述指標比較差異無統計學意義(P>0.05).結論 氟比洛芬酯靜脈註射後在大鼠手術切口部位肌肉組織中的分佈高于非手術側,提示氟比洛芬酯具有靶嚮鎮痛作用.
목적 평개불비락분지정맥주사후재대서수술절구부위기육조직중적분포.방법 청길급웅성SD대서40지,체중240～260 g,수궤분위5조(n=8):절구통조(IP조)화불동제량불비락분지조(F_(1～4)조).건립대서절구통모형후20 min시IP조경미정맥주사생리염수0.5 ml,F_(1～4)조경미정맥분별주사불비락분지2、4、8화16 mg/kg.우미정맥주사결속후2 h시,채용반상고효액상색보법측정기육불비락분함량,채용ELISA법측정전렬선소E_2 (PGE_2)함량.결과 여비수술측비교,F_(1～4)조수술측족저기육조직불비락분화PGE_2함량、IP조수술측PGE_2함량승고(P<0.01);여IP조비교,F_(1～4)조수술측족저기육조직PGE_2함량강저(P<0.05),F_4조비수술측족저기육조직PGE_2함량강저,F_(1～3)조비수술측상술지표차이무통계학의의(P>0.05);F_1조、F_2조、F_3조화F_4조수술측족저기육조직불비락분화PGE_2함량의차승고,F_4조비수술측족저기육조직불비락분화PGE_2함량고우F_1조、F_2조화F_3조(P<0.05혹0.01),F_1조、F_2조화_3조비수술측상술지표비교차이무통계학의의(P>0.05).결론 불비락분지정맥주사후재대서수술절구부위기육조직중적분포고우비수술측,제시불비락분지구유파향진통작용.
Objective To determine the distribution of flurbiprofen axetil in the muscle tissue in the surgical field after intravenous administration in rats. Methods Forty pathogen-free male SD rats weighing 240-260 g were randomly divided into 5 groups (n=8 each) : Ⅰ control group (group C) and Ⅱ-Ⅴ flurbiprofen groups received iv flurbiprofen axetil 2, 4, 8, 16 mg/kg respectively (group F_1, F_2, F_3, F_4). An 1 cm long incision was made in the right plantar surface from the heel to the toes according to the method described by Brennen under isoflurane anesthesia. The muscle was cut through in the incision but not severed. Flurbiprofen axetil was injected iv via the vein in the tail at 20 min after plantar incision was made in group Ⅱ-Ⅴ . At 2 h after iv flurbiprofen axetil administration, the animals were anesthetized with intraperitoneal 3% pentobarbital 100 mg/kg. The muscles of both hindpaws were dissected, homogenized and centrifuged. The supernatant was separated for determination of flurbiprofen content (by RP-HPLC) and PGE_2 content ( by ELISA). Results The flurbiprofen content in the muscle of hindpaw on the operated side was significantly increased dose-dependently compared with that on the non-operated side and was significantly higher than that on the non-operated side. The flurbiprofen content in the muscle of hindpaw on the non-operated side was significantly higher in group F_4 than in the other 4 groups. There was no significant difference in the flurbiprofen content in the paw muscle on the non-operated side among group C, F_1 F_2 and F_3. The PGF_2 content was significantly higher in the hindpaw muscle on the operated side than that on the non-operated side, and was significantly decreased by flurbiprofen dose-dependently. Conclusion The content of flurbiprofen is significantly higher in the muscle tissue in the surgical field after iv administration than that on the non-operated side, indicating that flurbiprofen axtei has target-analgesic effect.
