CAJ | 학술논문

目的研究900 MHz微波辐射对γ射线致小鼠骨髓造血损伤的拮抗作用及其机制.方法 60Co γ射线一次性全身照射雄性健康昆明种小鼠,建立放射性造血损伤模型,观察不同剂量微波对小鼠γ射线照射后30d存活率及存活时间、体重、外周血象和脏器系数的影响,不同时相点小鼠骨髓粒-巨噬细胞系集落形成单位(CFU-GM)形成及骨髓有核细胞(BMNCs)增殖活性的变化,同时采用ELISA法检测血清中集落刺激因子(GM-CSF)、白细胞介素-3(IL-3)分泌水平.结果 60Co γ射线照射前给予120 μW/cm2的900 MHz微波辐射能明显提高受照射小鼠的存活率,促进外周血白细胞恢复,提高受照射小鼠的脾脏和胸腺系数,增强γ射线照射后,小鼠骨髓BMNCs增殖活性下降程度减小,并可促进CFU-GM集落形成,刺激GM-CSF、IL-3的表达.结论特定强度的微波辐射具有一定的辐射防护作用,其作用机制可能与刺激GM-CSF表达、促进BMNCs增殖分化、降低射线导致的造血干细胞/祖细胞(HSCs/HPCs)增殖抑制,加快造血系统的重建有关.
목적 연구900 MHz미파복사대γ사선치소서골수조혈손상적길항작용급기궤제.방법 60Co γ사선일차성전신조사웅성건강곤명충소서,건립방사성조혈손상모형,관찰불동제량미파대소서γ사선조사후30d존활솔급존활시간、체중、외주혈상화장기계수적영향,불동시상점소서골수립-거서세포계집락형성단위(CFU-GM)형성급골수유핵세포(BMNCs)증식활성적변화,동시채용ELISA법검측혈청중집락자격인자(GM-CSF)、백세포개소-3(IL-3)분비수평.결과 60Co γ사선조사전급여120 μW/cm2적900 MHz미파복사능명현제고수조사소서적존활솔,촉진외주혈백세포회복,제고수조사소서적비장화흉선계수,증강γ사선조사후,소서골수BMNCs증식활성하강정도감소,병가촉진CFU-GM집락형성,자격GM-CSF、IL-3적표체.결론 특정강도적미파복사구유일정적복사방호작용,기작용궤제가능여자격GM-CSF표체、촉진BMNCs증식분화、강저사선도치적조혈간세포/조세포(HSCs/HPCs)증식억제,가쾌조혈계통적중건유관.
Objective To investigate antagonistic effect of microwave on hematopoietic damage of mice induced by γ-ray irradiation. Methods Male healthy Kunning mice were treated with low dose mi-crowave radiation before exposure to 60Co γ-ray irradiation of 8.0 Gy. The 30-day survival rate and average sur-vival time of the mice after the treatment were examined. Peripheral blood parameters and the organ indexes of thymus and spleen were also observed in the irradiated mice. After exposure to 5.0 Gy gamma irradiation, in-dexes of hematopoietic loci formation of bone marrow cells (CFU-GM) and the proliferation activity of BMNCs were examined. The serum concentration of hemopoietic factors (GM-CSF and IL-3) were detected by ELISA kits. Results Pre-exposure with 120 μW/cm2 900 MHz microwave increased the 30-day survival rate (P< 0.05 ) and the number of white blood cells of γ-ray treated mice. The increases of the organ indexes of thymus and spleen, proliferation activity of BMNCs and CFU-GM hematopoietic foci numbers, as well as the higher serum concentration of GM-CSF and IL-3 were observed in the microwave pre-exposure group. Conclusion Low dose microwave radiation may exert potential antagonistic effects on hematopoietic injuries induced by ionizing radiation. The underlying mechanisms might be related with stimulation of hematopoietic growth fac-tors expression, promotion of HSCs/HPCs proliferation, suppression on the reduction of HSCs/HPCs caused by 60Co γ-ray, and enhanced construction of the hematopoietic system.