CAJ | 학술논문

目的探讨锌指蛋白A20抑制NF -κB表达对早期脊髓损伤的影响.方法健康雌性C57/BL/6J小鼠随机分成治疗组、对照组和假手术组,建立小鼠脊髓损伤模型,损伤部位即刻应用A20后,采用RT -PCR法和Western blot法检测A20表达的变化,Western blot法检测NF - κB表达的变化；TUNEL法检测脊髓损伤区凋亡细胞数；BMS法评价脊髓功能的恢复.结果 A20治疗后1h,在治疗局部的脊髓组织内即可检测到A20表达,表达呈逐渐增强趋势；而NF - κB表达量相应逐步降低；治疗后1d,损伤区域内的TUNEL(+)细胞数开始减少,治疗后3周,阳性细胞数显著减少.BMS评分在治疗后1周开始改善,在2、3周,显著高于对照组(P＜0.05).结论 A20可以抑制NF - κB的表达,减少脊髓损伤后的细胞凋亡,促进受损脊髓的功能改善.
목적 탐토자지단백A20억제NF -κB표체대조기척수손상적영향.방법 건강자성C57/BL/6J소서수궤분성치료조、대조조화가수술조,건립소서척수손상모형,손상부위즉각응용A20후,채용RT -PCR법화Western blot법검측A20표체적변화,Western blot법검측NF - κB표체적변화；TUNEL법검측척수손상구조망세포수；BMS법평개척수공능적회복.결과 A20치료후1h,재치료국부적척수조직내즉가검측도A20표체,표체정축점증강추세；이NF - κB표체량상응축보강저；치료후1d,손상구역내적TUNEL(+)세포수개시감소,치료후3주,양성세포수현저감소.BMS평분재치료후1주개시개선,재2、3주,현저고우대조조(P＜0.05).결론 A20가이억제NF - κB적표체,감소척수손상후적세포조망,촉진수손척수적공능개선.
Objective To investigate the effects of A20 suppressing NF - κB expression on spinal cord injury at early stage.Methods Female C57/BL/6J mice was randomly divided into treatment group,control group and sham group.Immediately after compression lesion in mouse spinal cord injury model,A20was applied to the damaged tissue by lipsome. RT - PCR and Western blot were employed to detect the expression of A20a ndWestern blot to detect NF - κB.TUNEL method was used to detecte the aptosis of neurocytes in the injuried spinal area.Behavioural recovery was assessed using Bassl Mouse Scale.Results 1hour after treatment,A20 expression appeared in leisoned tissue and gradually increased,resulted in steady decline of NF - κB activation.The number of TUNEL( + ) cells in injuried region began to decrease 1day after treatment and significantly in 3 weeks.Compared with control group,BMS of treatment group increased 1week after treatment and significantly in 2 and 3 weeks( P ＜ 0.05 ).Conclusions A20 could suppress the NF - κB expression,which would diminish the neural apoptosis and promote funtional recovery after spinal cord injury.