CAJ | 학술논문

目的探讨大鼠局灶性脑缺血再灌注后凋亡诱导因子(AIF)的表达变化及与细胞凋亡的关系.方法将Wistar大鼠分为假手术组(6只)和模型组(30只).模型组大鼠采用线栓法建立大脑中动脉闭塞模型,假手术组大鼠插线较模型组浅,不造成大脑中动脉闭塞.观察假手术组及模型组缺血再灌注后6 h、24 h、48 h、72 h和7 d缺血半暗带AIF的表达,同时利用TUNEL法观察对应区域细胞凋亡的动态变化规律.结果模型组脑缺血再灌注6 h在缺血半暗带区AIF阳性细胞显著增加,再灌注48 h达到高峰[(130.47±11.32)个];各时相点均可见细胞凋亡,凋亡细胞数以再灌注48 h最多f(118.53±11.67)个];各组问比较差异均有统计学意义(JP<0.05).结论局灶性脑缺血再灌注可致AIF表达增加.并引起细胞凋亡.
목적 탐토대서국조성뇌결혈재관주후조망유도인자(AIF)적표체변화급여세포조망적관계.방법 장Wistar대서분위가수술조(6지)화모형조(30지).모형조대서채용선전법건립대뇌중동맥폐새모형,가수술조대서삽선교모형조천,불조성대뇌중동맥폐새.관찰가수술조급모형조결혈재관주후6 h、24 h、48 h、72 h화7 d결혈반암대AIF적표체,동시이용TUNEL법관찰대응구역세포조망적동태변화규률.결과모형조뇌결혈재관주6 h재결혈반암대구AIF양성세포현저증가,재관주48 h체도고봉[(130.47±11.32)개];각시상점균가견세포조망,조망세포수이재관주48 h최다f(118.53±11.67)개];각조문비교차이균유통계학의의(JP<0.05).결론 국조성뇌결혈재관주가치AIF표체증가.병인기세포조망.
Objective To study the relationship between the expression of apoptosis inducing factor (AIF) and neuronal apoptosis in rats with focal ischemia reperfusion.Methods Wistar rats were divided into sham-operated (n=6) and model (n=30) groups.Middle cerebral artery occlusion (MCAO)models were established by thread ligation.The expression of AIF in the ischemic penumbra was observed in the sham-operated and model groups 6 h and 1,2,3 and 7 d after the reperfusion; the changes of neuronal apoptosis in corresponding region were observed by TUNEL staining at the same time.Results AIF-positive cells in the ischemic penumbra were significantly increased in the model group 6 h after the ischemic reperfusion,reaching its peak level at 48 h of reperfusion (130.47±11.32 cells).Cell apoptosis occurred at each time point of ischemic reperfusion and the percentage of apoptosis cells at 48 h ofreperfusion (118.53±11.67 ceils) was the highest among all the time points.Significant differences between the 2 groups were found between each 2 time points (P<0.05).Conclusion Focal ischemia reperfusion can induce the increased expression of AIF positive cells and neuronal apoptosis.