CAJ | 학술논문

目的探讨OX40基因(TNFRSF4)rs2298212G/A位点与山东汉族人群冠状动脉粥样硬化疾病的相关性.方法在山东大学齐鲁医院心内科和健康体检中心分别收集到冠状动脉粥样硬化疾病患者536例和正常对照544名,采用聚合酶链反应-限制性片段长度多态性方法对OX40基因rs2298212G/A多态性位点进行基因分型,并对数据进行统计分析.结果基因型与等位基因频率分布在病例组与对照组之间差异均无统计学意义(P＞0.05).在回归校正了年龄、性别、体重指数、收缩压、舒张压、血糖、总胆固醇及甘油三酯等因素的影响后,基因型频率分布差异仍无统计学意义(P＞0.05).在对冠状动脉受累支数进行的分层分析发现,受累1支与受累3支之间,基因型与等位基因频率分布差异均有统计学意义(P＜0.05).结论 OX40基因rs2298212G/A多态位点同山东汉族人群冠状动脉粥样硬化疾病之间无关联性存在,但该位点可能与冠状动脉粥样硬化的严重程度相关.
목적 탐토OX40기인(TNFRSF4)rs2298212G/A위점여산동한족인군관상동맥죽양경화질병적상관성.방법 재산동대학제로의원심내과화건강체검중심분별수집도관상동맥죽양경화질병환자536례화정상대조544명,채용취합매련반응-한제성편단장도다태성방법 대OX40기인rs2298212G/A다태성위점진행기인분형,병대수거진행통계분석.결과 기인형여등위기인빈솔분포재병례조여대조조지간차이균무통계학의의(P＞0.05).재회귀교정료년령、성별、체중지수、수축압、서장압、혈당、총담고순급감유삼지등인소적영향후,기인형빈솔분포차이잉무통계학의의(P＞0.05).재대관상동맥수루지수진행적분층분석발현,수루1지여수루3지지간,기인형여등위기인빈솔분포차이균유통계학의의(P＜0.05).결론 OX40기인rs2298212G/A다태위점동산동한족인군관상동맥죽양경화질병지간무관련성존재,단해위점가능여관상동맥죽양경화적엄중정도상관.
Objective To study the association of the OX40 gene rs2298212G/A polymorphism with coronary atherosclerotic disease (CAD) in Chinese Han population. Methods Five hundred and thirty six CAD patients and 544 age and ethnic matched controls of Chinese Hah population were recruited from Qilu Hospital, Shandong University. Polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) was used to genotype the selected single nucleotide polymorphism. Distributions of genotypic and allelic frequencies were analyzed by Chi square test. Results The distribution of genotypic and allelic frequencies have no significant differences between the CAD cases and controls (P＞0.05), even after adjusting for age, gender, body mass index, systolic blood pressure, diastolic blood pressure, glucose, total cholesterol, and triglyceride. However, when substratification analysis of the involved coronary artery vessels was performed, significant difference was found between single-vessel and triple-vessel (P=0.02,OR=1.56,95%CI:1.08-2.26) involvement. Conclusion The rs2298212G/A polymorphism in OX40 gene may be associated with the severity of coronary atherosclerotic disease.