CAJ | 학술논문

以地高辛为标记物，利用PCR方法制备16SrRNA基因探针与ctx A基因探针，经Southern杂交，对霍乱弧菌进行分型。对广东地区分离自病人的60株埃尔托型(EVC)、10株O139群和2株标准株进行研究。经16SrRNA基因在探针共分为4个类型(A、B、C和D)，EVC和O139群均以D型为优势克隆。经ctxA基因探针Southern杂交，共分为A和B型，2株分离自1998年的EVC为A型，其余EVC和O139群均为B型。与国内外资料比较，广东地区霍乱弧菌的优势克隆与国内其它地区及东南亚地区的基本一致。
이지고신위표기물，이용PCR방법제비16SrRNA기인탐침여ctx A기인탐침，경Southern잡교，대곽란호균진행분형。대엄동지구분리자병인적60주애이탁형(EVC)、10주O139군화2주표준주진행연구。경16SrRNA기인재탐침공분위4개류형(A、B、C화D)，EVC화O139군균이D형위우세극륭。경ctxA기인탐침Southern잡교，공분위A화B형，2주분리자1998년적EVC위A형，기여EVC화O139군균위B형。여국내외자료비교，엄동지구곽란호균적우세극륭여국내기타지구급동남아지구적기본일치。
Vibrio cholerae strains were differnetiated by using 16SrRNAgene probe and ctx A gene probe. Two probes were developed byPCR method with DIG molecular. 60 E1Tor(EVC) and 10 O139strains isolated form patients in Guangdong province and 2 re-frence strains were analysed. By using 16SrRNA gene probe,strains were divided into 4 types(A、B、C and D), and most strainswere type B. By using ctx A gene probe, strains were divided into2 types, and type B was predominant type. Predominant types ofstrains isolated from Guangdong province cooperated with those ofstrains from other districts of China and South east of Asia.