CAJ | 학술논문

以紫茎泽兰不同发育时期花蕾为实验材料,通过光学显微观察、DNA ladder检测表明紫茎泽兰花蕾发育过程中绒毡层有PCD现象发生,自紫茎泽兰花蕾中克隆长为679 bp的Beclin1cDNA基因部分片段,同烟草叶片的Beclin1基因序列(AY701316)同源性为98%,通过Northern blotting分析,在紫茎泽兰花蕾发育过程中细胞程序性死亡(PCD)相关Beclin1基因表达在花蕾发育第Ⅱ期和Ⅲ期较为旺盛,且在花蕾发育第Ⅱ期最强烈.本研究首次克隆并证实紫茎泽兰花蕾Beclin1基因与PCD有一定的相关性,为分析紫茎泽兰入侵机理提供新的思路和手段.
이자경택란불동발육시기화뢰위실험재료,통과광학현미관찰、DNA ladder검측표명자경택란화뢰발육과정중융전층유PCD현상발생,자자경택란화뢰중극륭장위679 bp적Beclin1cDNA기인부분편단,동연초협편적Beclin1기인서렬(AY701316)동원성위98%,통과Northern blotting분석,재자경택란화뢰발육과정중세포정서성사망(PCD)상관Beclin1기인표체재화뢰발육제Ⅱ기화Ⅲ기교위왕성,차재화뢰발육제Ⅱ기최강렬.본연구수차극륭병증실자경택란화뢰Beclin1기인여PCD유일정적상관성,위분석자경택란입침궤리제공신적사로화수단.
It was found in the present study that programmed cell death (PCD) took place during the tapetum degenerating and pollen developing by microscopical observation and DNA ladder detection in Eupatorium adenophorum buds. Simultaneously, Beclin1 -like gene was cloned from E. adenophorum buds at four different growth stages by RT-PCR method. The partial Beclin1 -like gene (679 bp) was 98% similar to that of tobacco leaves (AY701316). Northern blotting showed that the expression of Beclin1 -like gene in the stage Ⅱ was the strongest. The Beclin1 -like gene was cloned in E. adenophorum buds for the first time and some relationships between the Beclin1 -like Gene and PCD during the procreation of E. adenophorum were demonstrated. In addition, the results provided a new idea to further explore the invading mechanism of E. adenophorum.