CAJ | 학술논문

由于缺乏详尽的热力学参数,基于网络拓扑的代谢途径分析是现阶段最重要的途径分析方法.首先简单地介绍了基于网络的途径分析方法发展简史,随后着重说明了基于凸分析的两种代谢途径分析方法:基元模式和极端途径及它们之间的异同,并详细阐述了这两种方法的具体应用.最后,使用极端途径分析了苏云金杆茵的PHB代谢.结果表明,除了传统的途径,还存在一些新颖的途径可用于PHB合成.
유우결핍상진적열역학삼수,기우망락탁복적대사도경분석시현계단최중요적도경분석방법.수선간단지개소료기우망락적도경분석방법발전간사,수후착중설명료기우철분석적량충대사도경분석방법:기원모식화겁단도경급타문지간적이동,병상세천술료저량충방법적구체응용.최후,사용겁단도경분석료소운금간인적PHB대사.결과표명,제료전통적도경,환존재일사신영적도경가용우PHB합성.
Due to the absence of detailed kinetic parameters,one of the most important methods,network topology based metabolic pathway analysis has been developed.The paper firstly introduces the brief history of network based on pathway analysis.Then,two such network based methods are emphasized:Elementary modes and extreme pathways,with theft similarities,differences and ap-plications.At last,extreme pathways are employed to analyze PHB metabolism of B.thuringiensis.Exeept for "historical" biochemical pathways,the results also suggest that there are some novel pathways.