中华骨科杂志
中華骨科雜誌
중화골과잡지
CHINESE JOURNAL OF ORTHOPAEDICS
2008年
8期
673-677
,共5页
任长乐%吕德成%纪军%赵俊君
任長樂%呂德成%紀軍%趙俊君
임장악%려덕성%기군%조준군
脊髓损伤%巨噬细胞%移植
脊髓損傷%巨噬細胞%移植
척수손상%거서세포%이식
Spinal cord injuries%Macrophages%Transplantation
目的 探讨活性巨噬细胞移植对脊髓损伤修复的促进作用.方法 用改良Allen法制成大鼠脊髓损伤模型.随机分成对照组(A组),脊髓内微量注射生理盐水20μl;活性巨噬细胞移植组(B组),脊髓内注射活性巨噬细胞悬液20μl.移植后7、14、28 d采用斜板试验、脊髓运动功能BBB(Basse,Beattie and Bresnahan)评分法观察大鼠运动功能恢复情况,脊髓诱发电位检测观察神经功能恢复,HE染色观察脊髓损伤处空洞面积的改变情况,免疫组化法观察移植的活性巨噬细胞的存活情况及损伤部位神经纤维的再生情况.结果 术后28 d,两组斜板倾斜角度差异有统计学意义(A组44.96°±5.70°,B组52.72°±6.51°,P＜0.05);两组BBB评分差异有统计学意义(A组6.8±1.2,B组11.8±2.2,P＜0.05).同时,两组MEP潜伏期差异也有统计学意义[A组(4.69±0.47)mg,B组(3.62±1.29)ms,P＜0.05],两组SEP潜伏期差异有统计学意义[A组(4.19±1.97)ms,B组(2.01±0.55)ms,P＜0.05].两组神经轴突计数差异有统计学意义[A组(32.8±6.1)条/mm2,B组(40.8±9.0)条//mm2 P＜0.05].实验组可见损伤区巨噬细胞存在,脊髓损伤处空洞面积减小,有明显神经纤维再生.结论 活性巨噬细胞可在脊髓损伤处存活并减轻脊髓损伤,减小脊髓损伤处空洞面积,促进受损轴突的再生和运动功能的恢复.
目的 探討活性巨噬細胞移植對脊髓損傷脩複的促進作用.方法 用改良Allen法製成大鼠脊髓損傷模型.隨機分成對照組(A組),脊髓內微量註射生理鹽水20μl;活性巨噬細胞移植組(B組),脊髓內註射活性巨噬細胞懸液20μl.移植後7、14、28 d採用斜闆試驗、脊髓運動功能BBB(Basse,Beattie and Bresnahan)評分法觀察大鼠運動功能恢複情況,脊髓誘髮電位檢測觀察神經功能恢複,HE染色觀察脊髓損傷處空洞麵積的改變情況,免疫組化法觀察移植的活性巨噬細胞的存活情況及損傷部位神經纖維的再生情況.結果 術後28 d,兩組斜闆傾斜角度差異有統計學意義(A組44.96°±5.70°,B組52.72°±6.51°,P＜0.05);兩組BBB評分差異有統計學意義(A組6.8±1.2,B組11.8±2.2,P＜0.05).同時,兩組MEP潛伏期差異也有統計學意義[A組(4.69±0.47)mg,B組(3.62±1.29)ms,P＜0.05],兩組SEP潛伏期差異有統計學意義[A組(4.19±1.97)ms,B組(2.01±0.55)ms,P＜0.05].兩組神經軸突計數差異有統計學意義[A組(32.8±6.1)條/mm2,B組(40.8±9.0)條//mm2 P＜0.05].實驗組可見損傷區巨噬細胞存在,脊髓損傷處空洞麵積減小,有明顯神經纖維再生.結論 活性巨噬細胞可在脊髓損傷處存活併減輕脊髓損傷,減小脊髓損傷處空洞麵積,促進受損軸突的再生和運動功能的恢複.
목적 탐토활성거서세포이식대척수손상수복적촉진작용.방법 용개량Allen법제성대서척수손상모형.수궤분성대조조(A조),척수내미량주사생리염수20μl;활성거서세포이식조(B조),척수내주사활성거서세포현액20μl.이식후7、14、28 d채용사판시험、척수운동공능BBB(Basse,Beattie and Bresnahan)평분법관찰대서운동공능회복정황,척수유발전위검측관찰신경공능회복,HE염색관찰척수손상처공동면적적개변정황,면역조화법관찰이식적활성거서세포적존활정황급손상부위신경섬유적재생정황.결과 술후28 d,량조사판경사각도차이유통계학의의(A조44.96°±5.70°,B조52.72°±6.51°,P＜0.05);량조BBB평분차이유통계학의의(A조6.8±1.2,B조11.8±2.2,P＜0.05).동시,량조MEP잠복기차이야유통계학의의[A조(4.69±0.47)mg,B조(3.62±1.29)ms,P＜0.05],량조SEP잠복기차이유통계학의의[A조(4.19±1.97)ms,B조(2.01±0.55)ms,P＜0.05].량조신경축돌계수차이유통계학의의[A조(32.8±6.1)조/mm2,B조(40.8±9.0)조//mm2 P＜0.05].실험조가견손상구거서세포존재,척수손상처공동면적감소,유명현신경섬유재생.결론 활성거서세포가재척수손상처존활병감경척수손상,감소척수손상처공동면적,촉진수손축돌적재생화운동공능적회복.
Objective To investigate the promoting effects of activated macrophages transplantation on function recovery of rat spinal cord injuries(SCI).Methods The spinal cord of adult rats was injured with modified Allen method,and the rats were randomly divided into 2 groups.In one group(A group),slight saline solution(20 μl)was injected into the SCI site,while in the other group(B group)activated macrophages(20 μl)were transplanted instead.The inclined plane test and Basso,Beattie and Bresnahan locomotor rating scale(BBB scale)were used to observe the recovery of rats' behavioral function on 7,14 and 28 d after the transplantation.while spinal evoked potential(SEP) tests were used to observe the recovery of spinal cord nervousness function.HE staining to reflect the change of cavity volume.and immunohistochemical method to observe the survival of activated macrophages transplantation and regeneration of damaged nerve fibers.Results On the 28th day after transplantation,inclined plane critical point was significantly different between group A and B.which were 44.96°±5.70°and 52.72°±6.51°respectively;BBB scale had significant difference between group A and group B,which were 6.8±1.2 and 11.8±2.2;MEP and SEP latencies had significant differences.The MEP latencies of group A and group B were(4.69±0.47)ms and(3.62±1.29)ms,respectively and the SEP latencies of group A and group B were(4.19±1.97)ms and(2.01±0.55)ms respectively.On the 28th day after transplantation.nerves axons count(strip/mm2)was significantly different between group A and group B,which were 32.8±6.1 and 40.8±9.0 respectively.In B group,macrophages survived in the iniury area,cavity volume of the injured site was significantly reduced and there was significant regeneration of nerve fiber.Conclusion Activated macrophages can survive in the iniury site to relieve SCI.reduce cavity volume and promote axonal regeneration and improve functional recovery.
