CAJ | 학술논문

目的观察氡及其子体暴露对小鼠肺组织P53蛋白表达及基因突变的影响.方法建立氡染毒小鼠模型,使染毒累积剂量分别为30和60工作水平月(WLM);采用TUNEL法,检测小鼠肺组织细胞凋亡程度;采用免疫组化S-P法、Western-b1ot法及实时荧光定量PCR法,检测各组肺组织P53蛋白表达情况;采用PCR-SSCP法检测p53基因的突变情况.结果与正常对照组相比,氡染毒30和60 WLM小鼠肺细胞凋亡指数均明显增加(t=18.11、-10.30,P＜0.05);氡染毒30和60 WLM组P53蛋白亦明显增加(t=-11.08、-7.00,P＜0.05).但整个实验未观察到p53基因突变.结论 p53与氡染毒诱发小鼠肺损伤密切相关.
목적 관찰동급기자체폭로대소서폐조직P53단백표체급기인돌변적영향.방법 건립동염독소서모형,사염독루적제량분별위30화60공작수평월(WLM);채용TUNEL법,검측소서폐조직세포조망정도;채용면역조화S-P법、Western-b1ot법급실시형광정량PCR법,검측각조폐조직P53단백표체정황;채용PCR-SSCP법검측p53기인적돌변정황.결과 여정상대조조상비,동염독30화60 WLM소서폐세포조망지수균명현증가(t=18.11、-10.30,P＜0.05);동염독30화60 WLM조P53단백역명현증가(t=-11.08、-7.00,P＜0.05).단정개실험미관찰도p53기인돌변.결론 p53여동염독유발소서폐손상밀절상관.
Objective To explore the effect of radon and its progeny on the expression and mutations of p53 in lung tissue of mouse model. Methods Apoptosis was detected by terminal deoxynucleotidy transferase-mediated dUTP-biotin nick end labeling. The expression of p53 gene was analyzed by immunohistochemistry, Western blot and realtime-PCR. PCR-SSCP was used to detect the mutation of p53 in lung tissues. Results Compared with those in the control group, the apoptotic index were increased significantly in 30 WLM and 60 WLM groups( t = 18.11, -10.30,P ＜ 0.05 ). The p53 protein was increased significantly ( t = -11.08, P ＜ 0.05; t = - 7.00, P ＜ 0.0 ) ) in 30 WLM and 60 WLM groups. The mutation of p53 gene was not detected in lungs of radon-exposure mice. Conclusions Lung and bronchus might be the targets of radon and its progeny, and p53 gene plays an important role in the progression of radon-induced lung injury.