CAJ | 학술논문

目的应用包裹阿柔比星的血管内皮生长因子(VEGF)的长循环脂质体(阿柔比星-VEGF-SSL)于体外靶向杀伤恶性黑素瘤细胞.方法通过体外结合试验,验证阿柔比星-VEGF-SSL对恶性黑素瘤A375细胞的特异结合能力.通过体外细胞毒试验(MTT),检测阿柔比星-VEGF-SSL对A375细胞增殖活性的影响.结果阿柔比星-VEGF-SSL可与A375细胞特异结合,结合率可达非特异性脂质体的2.15倍.阿柔比星-VEGF-SSL可特异抑制A375细胞的增殖活性.48 h细胞毒试验阿柔比星-VEGF-SSL抑制A375细胞增殖活性的作用与游离阿柔比星相似(P＞0.05),优于无VEGF的阿柔比星脂质体(阿柔比星-SSL)(P＜0.05),而对非靶细胞(黑素细胞)增殖活性的抑制作用弱于两者.0.5 h预处理试验表明:缩短药物与细胞接触时间后,阿柔比星-VEGF-SSL仍保持较强抑制A375细胞增殖活性的作用.结论阿柔比星-VEGF-SSL可特异性识别恶性黑素瘤A375细胞,并作为良好载体将阿柔比星带入瘤细胞,显著抑制A375细胞的增殖活性,实现其靶向杀伤作用.
목적 응용포과아유비성적혈관내피생장인자(VEGF)적장순배지질체(아유비성-VEGF-SSL)우체외파향살상악성흑소류세포.방법 통과체외결합시험,험증아유비성-VEGF-SSL대악성흑소류A375세포적특이결합능력.통과체외세포독시험(MTT),검측아유비성-VEGF-SSL대A375세포증식활성적영향.결과 아유비성-VEGF-SSL가여A375세포특이결합,결합솔가체비특이성지질체적2.15배.아유비성-VEGF-SSL가특이억제A375세포적증식활성.48 h세포독시험아유비성-VEGF-SSL억제A375세포증식활성적작용여유리아유비성상사(P＞0.05),우우무VEGF적아유비성지질체(아유비성-SSL)(P＜0.05),이대비파세포(흑소세포)증식활성적억제작용약우량자.0.5 h예처리시험표명:축단약물여세포접촉시간후,아유비성-VEGF-SSL잉보지교강억제A375세포증식활성적작용.결론 아유비성-VEGF-SSL가특이성식별악성흑소류A375세포,병작위량호재체장아유비성대입류세포,현저억제A375세포적증식활성,실현기파향살상작용.
Objective To evaluate the targeted killing of malignant melanoma cells by aclarubicin liposomes conjugated with vascular endothelial growth factor(ADM-VEGF-SSL)in vitro.Metheds To detect the binding abilitv of liposomes to malignant melanoma(MM)cells,the human malignant melanoma cell line A375 was cultured in the presence of ADM-VEGF-3H-SSL or ADM-3H-SSL for 2 days followed by the detection of radioactivity of these cells.Then.A375 cells were cultured with various concentrations(0.01,0.1,1,10,100 mol/L)of ADM-VEGF-SSL,ADM-SSL or free ADM for 48 hours in the 48-hour cytotoxity test,or for 0.5 hour followed by another 48-hour culture in drug-free medium in the 0.5-hour cytotoxity test.After that,MTT assay was used to detect the survival rate of these cells.Results ADM-VEGF-SSL could specifically bind to and kill A375 cells.The binding rate of ADM-VEGF-SSL was 2.15 folds as high as that of ADM-SSL.The survival rate of A375 cells after being treated with ADM-VEGF-SSL for 48 hour was similar to that with flee ADM(P＞0.05).but lower than that with ADM-SSL(P＜0.05),while the survival rate of melanocytes treated with ADM-VEGF-SSL was higher than that with free ADM or ADM-SSL(both P＜0.05).As shown by the 0.5-hour cytotoxity test.shortening the treatment course did not attenuate the effect of ADM-VEGF-SSL on A375 cells.Conclusions ADM-VEGF-SSL can specifically recognize A375 cells.efficiently deliver adriamycin into tumor cells,markedly inhibit the proliferation of A375 cells,and eventually,a targeted kill of these cells is realized.