CAJ | 학술논문

目的探讨缺氧诱导因子及其相关蛋白在肝母细胞瘤(hepatoblastoma,HB)中表达情况及其作用.方法选择1998年至2009年入住我院并接受手术的肝母细胞瘤患儿及胆道闭锁患儿各30例,分为三组:肝母细胞瘤肿瘤组织、距肿瘤组织边缘5 cm以外的正常肝脏组织以及胆道闭锁活检肝组织,肿瘤组中以PRETEXT分期系统,将肿瘤组分为低分组(Ⅰ组+Ⅱ组,10例)和高分组(Ⅲ组+Ⅳ组,20例).采用免疫组织化学染色方法检测HIF-1α、HIF-2α、VEGF、GLUT-1、TGF-α在上述标本中的表达情况,并且比较各组的表达情况差异.结果 5种蛋白的阳性表达均主要表现为细胞质内棕黄色颗粒.肿瘤组HIF-1α、HIF-2a、VEGF、GLUT-1、TGF-a表达明显高于其他两组(P<0.01).肿瘤高分组中HIF-1α及TGF-α的表达水平高于低分组(P<0.05),而HIF-2α、VEGF、GLUT-1的表达两者无显著差别.结论缺氧诱导因子及其相关蛋白在肝母细胞瘤中的表达均有增加,提示HIF可能在肝母细胞瘤的发生发展中起着重要作用,其中HIF-1α还可能是潜在的预后判断指标之一.
목적 탐토결양유도인자급기상관단백재간모세포류(hepatoblastoma,HB)중표체정황급기작용.방법 선택1998년지2009년입주아원병접수수술적간모세포류환인급담도폐쇄환인각30례,분위삼조:간모세포류종류조직、거종류조직변연5 cm이외적정상간장조직이급담도폐쇄활검간조직,종류조중이PRETEXT분기계통,장종류조분위저분조(Ⅰ조+Ⅱ조,10례)화고분조(Ⅲ조+Ⅳ조,20례).채용면역조직화학염색방법검측HIF-1α、HIF-2α、VEGF、GLUT-1、TGF-α재상술표본중적표체정황,병차비교각조적표체정황차이.결과 5충단백적양성표체균주요표현위세포질내종황색과립.종류조HIF-1α、HIF-2a、VEGF、GLUT-1、TGF-a표체명현고우기타량조(P<0.01).종류고분조중HIF-1α급TGF-α적표체수평고우저분조(P<0.05),이HIF-2α、VEGF、GLUT-1적표체량자무현저차별.결론 결양유도인자급기상관단백재간모세포류중적표체균유증가,제시HIF가능재간모세포류적발생발전중기착중요작용,기중HIF-1α환가능시잠재적예후판단지표지일.
Objective To investigate the expressions of hypoxia-inducible factors(HIF)and its related proteins in hepatoblastoma.Methods Between 1998 and 2009,30 patients who were diagnosed with hepatoblastoma and 30 patients with biliary atresia were recruited in this study.The specimens of the tumor group were collected from the hepatoblastoma tissue.The specimens of the first control group were the liver tissues which located more than 5 cm away from the borderline of the tumor.The specimens of the second control group were the liver tissues from the 30 patients with biliary atresia.Immunohistochemical staining was performed to examine the expression level of HIF-1α,HIF-2α,vascular endothelial growth factor(VEGF),glucose transporter-1(GT1),and transforming growth factor-alpha(TGF-α)in specimens.Results The positive stained proteins appeared as brown granules in cytoplasm.The expression levels of all the 5 proteins of the tumor group were significantly higher than those of the other two control groups(P<0.01).The expression level of HIT-1α and TGF-α of the high-grade tumors were significantly higher than those of the low-grade tumors(P<0.05).Conclusions The expressions of HIF-1α,HIF-2α,VEGF,GT1 and TGF-α increase in hepatoblastoma tissues.It indicates HIF may play a role in the carcinogenesis of hepatoblastoma.