CAJ | 학술논문

目的探讨外周血单核细胞免疫排斥相关基因的检测时心脏移植排斥反应的诊断价值.方法采取心脏移植术患者外周血,应用实时荧光定量RT-PCR技术,观察心脏移植术后不同时期外周血单个核细胞中与免疫排斥相关多个基因系统的mRNA表达水平,并与术前和正常组的基因表达水平对照.结果心脏移植术前外周血单个核细胞16种与免疫排斥相关候选基因的mRNA的相对表达量与正常对照组比较差异无统计学意义(P>0.05).初步筛选出与机体免疫排斥状态有更大相关性的7种基因,术后3个月内心脏功能稳定组较术前和正常对照组ITGA4、FKB、IL1R-2 mRNA表达水平上调,PF4、ITGAM、TGFβ1、RHOU表达水平降低.这与临床观察的移植术后1～3个月免疫排斥概率最大相吻合.结论检测外周血单个核细胞基因mRNA表达水平的荧光定量RT-PCR方法简便快速、特异、重复性好、可信度高,但在检测心脏移植排斥反应方面值得进一步深入研究.
목적 탐토외주혈단핵세포면역배척상관기인적검측시심장이식배척반응적진단개치.방법 채취심장이식술환자외주혈,응용실시형광정량RT-PCR기술,관찰심장이식술후불동시기외주혈단개핵세포중여면역배척상관다개기인계통적mRNA표체수평,병여술전화정상조적기인표체수평대조.결과 심장이식술전외주혈단개핵세포16충여면역배척상관후선기인적mRNA적상대표체량여정상대조조비교차이무통계학의의(P>0.05).초보사선출여궤체면역배척상태유경대상관성적7충기인,술후3개월내심장공능은정조교술전화정상대조조ITGA4、FKB、IL1R-2 mRNA표체수평상조,PF4、ITGAM、TGFβ1、RHOU표체수평강저.저여림상관찰적이식술후1～3개월면역배척개솔최대상문합.결론검측외주혈단개핵세포기인mRNA표체수평적형광정량RT-PCR방법 간편쾌속、특이、중복성호、가신도고,단재검측심장이식배척반응방면치득진일보심입연구.
Objective To construct the micro-invasive immune rejection monitoring methods with peripher-al blood mononuclear cell gene expression detection and evaluate the clinic rejection estimation value. Methods The SYBR Green Ⅰ was used as fluorescent dye and the GAPDH as house keeping gene control in the quantitatiun RT-PCR technique to observe the 16 immune rejection relative genes expression features after heart transplantation. results were also compared with that of the normal people. Results The 16 immune rejection relative genes expres-sion were no different between normal people and the transplantation recipients before surgery (P>0.05). After heart transplantation the expression of ITGA4, FKB, ILI R-2 up regulated and the level of PF4、ITGAM、TGFβ1、 RHOU down regulated. The results were similar with the clinic observation that the immune rejection often occurs in the first 3 months after heart transplantation. It implied that these 7 genes may play an important role in the acute im-mune rejection after transplantation. Conclusion The real time quantitation RT-PCR methods were constructed suc-cessfully to detect the multiple immune relative genes expression and is of chnic applicable.