中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
12期
1159-1163
,共5页
张娟娟%赵广录%张丽君%王峰%洪福昌%蓝丽娜%吴肖冰%陶小华%张春来%卢次勇%冯铁建
張娟娟%趙廣錄%張麗君%王峰%洪福昌%藍麗娜%吳肖冰%陶小華%張春來%盧次勇%馮鐵建
장연연%조엄록%장려군%왕봉%홍복창%람려나%오초빙%도소화%장춘래%로차용%풍철건
淋病%沙眼衣原体%巢式聚合酶链反应%基因分型%序列分析
淋病%沙眼衣原體%巢式聚閤酶鏈反應%基因分型%序列分析
임병%사안의원체%소식취합매련반응%기인분형%서렬분석
Gonorrhea%Chlamydia trachomatis%Nested-PCR%Genotyping%Sequencing analysis
目的 了解深圳市拟诊为淋病患者中泌尿生殖道沙眼衣原体的合并感染情况及其基因型分布和序列变异特点.方法 采集401例拟诊为淋病患者的泌尿生殖道分泌物样本,应用Roche Amplicor全自动核酸检测系统对样本进行淋球菌和沙眼衣原体双检,提取DNA,应用巢式聚合酶链反应(nested-PGR)扩增沙眼衣原体主要外膜蛋白基因(omp1)中的VS1～VS2片段,并对其进行序列测定,所获得的序列利用Mega4.0软件与标准参考株进行比对,分析确定其基因型及序列变异情况.结果 401例拟诊为淋病患者中淋球菌的感染率为82.3%(330/401),沙眼衣原体的感染率为24.2%(97/401),淋球菌和沙眼衣原体的合并感染率为21.7%(87/401).97份沙眼衣原体阳性样本中获得73份沙眼衣原体基因片段序列,共检出8个基因型,分别为E型(27.4%)、G/Ga型(23.3%)、D/Da型(16.4%)、F型(13.7%)、J型(11.0%)、H型(5.5%)、B和K型(各1.4%).序列分析发现3例(4.1%)菌株发生错义突变,分别为D/Da型、E型、G/Ga型;F型、H型、J型和K型序列虽多见碱基突变,但均为同义突变.结论淋病患者合并感染沙眼衣原体的比例较高,且泌尿生殖道沙眼衣原体的基因型以E、G/Ga、D/Da和F型为主.序列分析可以为泌尿生殖道沙眼衣原体的分子流行病学研究提供依据.
目的 瞭解深圳市擬診為淋病患者中泌尿生殖道沙眼衣原體的閤併感染情況及其基因型分佈和序列變異特點.方法 採集401例擬診為淋病患者的泌尿生殖道分泌物樣本,應用Roche Amplicor全自動覈痠檢測繫統對樣本進行淋毬菌和沙眼衣原體雙檢,提取DNA,應用巢式聚閤酶鏈反應(nested-PGR)擴增沙眼衣原體主要外膜蛋白基因(omp1)中的VS1～VS2片段,併對其進行序列測定,所穫得的序列利用Mega4.0軟件與標準參攷株進行比對,分析確定其基因型及序列變異情況.結果 401例擬診為淋病患者中淋毬菌的感染率為82.3%(330/401),沙眼衣原體的感染率為24.2%(97/401),淋毬菌和沙眼衣原體的閤併感染率為21.7%(87/401).97份沙眼衣原體暘性樣本中穫得73份沙眼衣原體基因片段序列,共檢齣8箇基因型,分彆為E型(27.4%)、G/Ga型(23.3%)、D/Da型(16.4%)、F型(13.7%)、J型(11.0%)、H型(5.5%)、B和K型(各1.4%).序列分析髮現3例(4.1%)菌株髮生錯義突變,分彆為D/Da型、E型、G/Ga型;F型、H型、J型和K型序列雖多見堿基突變,但均為同義突變.結論淋病患者閤併感染沙眼衣原體的比例較高,且泌尿生殖道沙眼衣原體的基因型以E、G/Ga、D/Da和F型為主.序列分析可以為泌尿生殖道沙眼衣原體的分子流行病學研究提供依據.
목적 료해심수시의진위임병환자중비뇨생식도사안의원체적합병감염정황급기기인형분포화서렬변이특점.방법 채집401례의진위임병환자적비뇨생식도분비물양본,응용Roche Amplicor전자동핵산검측계통대양본진행림구균화사안의원체쌍검,제취DNA,응용소식취합매련반응(nested-PGR)확증사안의원체주요외막단백기인(omp1)중적VS1～VS2편단,병대기진행서렬측정,소획득적서렬이용Mega4.0연건여표준삼고주진행비대,분석학정기기인형급서렬변이정황.결과 401례의진위임병환자중림구균적감염솔위82.3%(330/401),사안의원체적감염솔위24.2%(97/401),림구균화사안의원체적합병감염솔위21.7%(87/401).97빈사안의원체양성양본중획득73빈사안의원체기인편단서렬,공검출8개기인형,분별위E형(27.4%)、G/Ga형(23.3%)、D/Da형(16.4%)、F형(13.7%)、J형(11.0%)、H형(5.5%)、B화K형(각1.4%).서렬분석발현3례(4.1%)균주발생착의돌변,분별위D/Da형、E형、G/Ga형;F형、H형、J형화K형서렬수다견감기돌변,단균위동의돌변.결론임병환자합병감염사안의원체적비례교고,차비뇨생식도사안의원체적기인형이E、G/Ga、D/Da화F형위주.서렬분석가이위비뇨생식도사안의원체적분자류행병학연구제공의거.
Objective To understand the prevalence rate of genital Chlamydia trachomatis among a population with suspected-Neisseria gonorrhoeae infection,the distribution of Chlamydia trachomatis genotypes,assess changes in omp1 sequences among patients with Neisseria gonorrhoeae and Chlamydia trachomatis coinfections.Methods Four hundred and one swabs were collected.Chlamydia trachomatis and Neisseria gonorrhoeae were detected by Roche Amplicor System.DNA were extracted from those samples and were amplified by nested PCR.PCR products were sequencing and analyzed by software Mega4.0.Results The prevalence of genital Chlamydia trachomatis infection,Neisseria gonorrhoeae infection and coinfection with genital gonorrhoea and genital chlamydia were 82.3%,24.2% and 21.7% each.Eight genotypes were identified in 73 sequences,including E(27.4%),G/Ga(23.3%),D/Da(16.4%),F(13.7%),J (11.0%),H(5.5%),B and K(each 1.4%).Sequencing analysis showed that 3 cases(4.1%) had missense mutation,including genotype D/Da,E,G/Ga.Genotypes F,H,J and K were more variable,however,most of them were silent mutation.Conclusion The prevalence rate of genital Chlamydia trachomatis among a population with Neisseria gonorrhoeae infection was high.The most common genotypes were genotype E,G/Ga,D/Da and F; Sequencing analysis has provided a tool for the molecular epidemiology of genital Chlamydia trachomatis infections.
