CAJ | 학술논문

目的探讨软组织静脉畸形侵袭性与RhoC表达的相关性.方法收集侵袭性静脉畸形组织、非侵袭性静脉畸形组织及正常皮下组织,采用Western blot和逆转录-聚合酶链反应(RT-PCR)检测RhoC蛋白及mRNA的表达.培养静脉畸形内皮细胞(VM-EC),小干扰RNA (siRNA)转染后检测细胞侵袭行为.结果 RhoC在侵袭组表达高于其他两组(P＜0.05),蛋白相对表达量分别为0.383±0.055、0.529±0.134、0.939±0.051,mRNA相对表达量为0.117 ±0.029、0.378±0.130、0.704 ±0.152.RNA干扰后,VM-EC侵袭能力和成血管能力均下降(P＜0.05),干扰前后穿透Matrigel胶的细胞数分别为56 ±6和20 ±4,形成完整血管环数分别为17 ±2和7±2.结论 RhoC是静脉畸形侵袭性发展中的重要蛋白,调控RhoC表达可影响其侵袭性进展.
목적 탐토연조직정맥기형침습성여RhoC표체적상관성.방법 수집침습성정맥기형조직、비침습성정맥기형조직급정상피하조직,채용Western blot화역전록-취합매련반응(RT-PCR)검측RhoC단백급mRNA적표체.배양정맥기형내피세포(VM-EC),소간우RNA (siRNA)전염후검측세포침습행위.결과 RhoC재침습조표체고우기타량조(P＜0.05),단백상대표체량분별위0.383±0.055、0.529±0.134、0.939±0.051,mRNA상대표체량위0.117 ±0.029、0.378±0.130、0.704 ±0.152.RNA간우후,VM-EC침습능력화성혈관능력균하강(P＜0.05),간우전후천투Matrigel효적세포수분별위56 ±6화20 ±4,형성완정혈관배수분별위17 ±2화7±2.결론 RhoC시정맥기형침습성발전중적중요단백,조공RhoC표체가영향기침습성진전.
Objective To investigate the correlation of the invasiveness of venous malformations and the expression of RhoC.Methods Specimens of invasive venous malformation tissues,non-invasive venous malformation tissues and normal subcutaneous tissues were collected.The expression of RhoC protein and mRNA was detected by Western blotting and polymerase chain reaction (PCR) respectively.RhoC-small interfering RNA (siRNA) was constructed and transfected into venous malformations endothelial cells,and invasion and angiogenesis were detected by invasion assay and endothelial cell network formation assay respectively.Results In the invasive venous malformation tissues,the expression levels of RhoC protein and mRNA were significantly higher than the others ( P ＜ 0.05 ).siRNA targeting RhoC inhibited vascular malformation cell invasion and angiogenesis (P ＜ 0.05 ).The number of cells which penetrated the Matrigel gelatin was (56 ± 6) vs (20 ± 4).The number of the integrity vascular ring formed was ( 17 ±2) vs (7 ±2).Conclusion RhoC may be involved in the invasive development of the venous malformation.Regulating RhoC may have influence on its invasion.