中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
7期
1026-1028
,共3页
滑膜干细胞%壳聚糖-明胶支架%组织工程%关节软骨
滑膜榦細胞%殼聚糖-明膠支架%組織工程%關節軟骨
활막간세포%각취당-명효지가%조직공정%관절연골
Synovium-derived mesenchymal stem cells%Chitosan-gelatin scaffold%Tissue engineering%Articular cartilage
目的 检测壳聚糖-明胶支架与人滑膜干细胞(SMSCs)的相容性.方法 接种滑膜干细胞于壳聚糖-明胶支架上,于第3、7和10天进行扫描电镜观察和噻唑蓝(MTT)分析.结果 扫描电镜显示支架上有大量滑膜干细胞生长,且细胞形态与对照组无明显差异,MTT分析显示支架组的A值关系为10d(1.43±0.11)≥7d(1.35±0.08)＞3d(0.43±0.08),且第7、10天组的A值明显大于同期对照组(0.64±0.15、0.59±0.13)(P＜0.05).结论 壳聚糖-明胶支架与滑膜干细胞的相容性良好.
目的 檢測殼聚糖-明膠支架與人滑膜榦細胞(SMSCs)的相容性.方法 接種滑膜榦細胞于殼聚糖-明膠支架上,于第3、7和10天進行掃描電鏡觀察和噻唑藍(MTT)分析.結果 掃描電鏡顯示支架上有大量滑膜榦細胞生長,且細胞形態與對照組無明顯差異,MTT分析顯示支架組的A值關繫為10d(1.43±0.11)≥7d(1.35±0.08)＞3d(0.43±0.08),且第7、10天組的A值明顯大于同期對照組(0.64±0.15、0.59±0.13)(P＜0.05).結論 殼聚糖-明膠支架與滑膜榦細胞的相容性良好.
목적 검측각취당-명효지가여인활막간세포(SMSCs)적상용성.방법 접충활막간세포우각취당-명효지가상,우제3、7화10천진행소묘전경관찰화새서람(MTT)분석.결과 소묘전경현시지가상유대량활막간세포생장,차세포형태여대조조무명현차이,MTT분석현시지가조적A치관계위10d(1.43±0.11)≥7d(1.35±0.08)＞3d(0.43±0.08),차제7、10천조적A치명현대우동기대조조(0.64±0.15、0.59±0.13)(P＜0.05).결론 각취당-명효지가여활막간세포적상용성량호.
Objective To investigate the compatibility between chitosan-gelatin scaffold and synovium-derived mesenchymal stem cells (SMSCs).Methods The SMSCs were obtained with passage isolation, and then seeded on chitosan-gelatin scaffold. Cytocompatibility was detected by using scanning electron microscopy (SEM) and methyl thiazol tetrazolium (MTT) assay at day 3, 7 and 10 after seeding.Results SEM image of scaffold showed that SMSCs spread well and homogenously distributed throughout the entire scaffold. MTT assay revealed that the cell viability in 3D scaffolds was as follows: (1.43±0.11) at day 10≥ (1.35±0.08) at day 7 > (0.43±0.08) at day 3, and at day 7 and 10, it was significantly higher than that in controls (day 7: 0.64±0.15, and day 10: 0.59±0.13;P＜0.05).Conclusion The chitosan-gelatin scaffold is compatible with SMSCs.
