中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2001年
3期
241-244
,共4页
孙林光%管锦霞%黄劭%余清声
孫林光%管錦霞%黃劭%餘清聲
손림광%관금하%황소%여청성
蛇毒液类%血浆%蛋白质C%酶激活
蛇毒液類%血漿%蛋白質C%酶激活
사독액류%혈장%단백질C%매격활
目的：从9种国产蛇毒中筛选具有激活血浆蛋白C作用的蛇毒。方法：运用活化部分凝血活酶时间（APTT）和发色底物实验分别观察抗凝活性和酰胺酶活性，综合抗凝活性和酰胺酶活性确定蛋白C蛇毒激活作用。结果：在9种国产蛇毒中烙铁头蛇毒及蝮蛇毒在1.5 mg/L浓度下即使人血浆纯蛋白C产生酰胺酶活性，并使APTT显著延长。结论：9种国产蛇毒中烙铁头蛇毒及蝮蛇毒具有激活人体血浆蛋白C成为活化蛋白C（APC）的作用。
目的：從9種國產蛇毒中篩選具有激活血漿蛋白C作用的蛇毒。方法：運用活化部分凝血活酶時間（APTT）和髮色底物實驗分彆觀察抗凝活性和酰胺酶活性，綜閤抗凝活性和酰胺酶活性確定蛋白C蛇毒激活作用。結果：在9種國產蛇毒中烙鐵頭蛇毒及蝮蛇毒在1.5 mg/L濃度下即使人血漿純蛋白C產生酰胺酶活性，併使APTT顯著延長。結論：9種國產蛇毒中烙鐵頭蛇毒及蝮蛇毒具有激活人體血漿蛋白C成為活化蛋白C（APC）的作用。
목적：종9충국산사독중사선구유격활혈장단백C작용적사독。방법：운용활화부분응혈활매시간（APTT）화발색저물실험분별관찰항응활성화선알매활성，종합항응활성화선알매활성학정단백C사독격활작용。결과：재9충국산사독중락철두사독급복사독재1.5 mg/L농도하즉사인혈장순단백C산생선알매활성，병사APTT현저연장。결론：9충국산사독중락철두사독급복사독구유격활인체혈장단백C성위활화단백C（APC）적작용。
AIM:To determine which species of snake venoms contained protein c activator among 9 species of Chinese snake venoms. METHODS: Anticoagulant activity was examined by activated partial thromboplastin time (APTT) assay，and amidolytic activity was measured with activated protein c (APC) specific chromogenic peptide substrate-chromozy APC. RESULTS: Among 9 species of Chinese snake venoms，Trimeresurus mucrosquamatus venom and Agkistrodon halys venom were not only able to generate amidolytic activity from purified human PC, but also prolonged APTT strongly even at such a concentration as 1.5 mg/L.CONCLUSION: Trimeresurus mucrosquamatus venom and Agkistrodon halys venom contain protein c activator which activating human plasma PC into APC.
