CAJ | 학술논문

利用Ty1/copia类反转录转座子的保守位点设计简并引物,从绿豆(Vigna radiata(L.)Wilczek)基因组中扩增得到了反转录转座子的逆转录酶序列.对扩增得到的约270bp的片段进行分离和克隆,并随机挑选了40个克隆进行测序,结果得到了36个单独的核酸序列,其中18个含有移码突变或终止子.根据序列比对,这些克隆可分为9组以及单个的9种.这40个克隆中,核酸序列相似性从8%到100%,显示出其核酸序列的高度异质性.将这些克隆的核酸序列与来自其他种植物的相应序列进行谱系分析,发现有些克隆与来自其他种植物的相应序列的亲缘关系比这些克隆之间更为接近.斑点杂交显示Ty1/copia反转录转座子约占绿豆基因组的9.3%.
이용Ty1/copia류반전록전좌자적보수위점설계간병인물,종록두(Vigna radiata(L.)Wilczek)기인조중확증득도료반전록전좌자적역전록매서렬.대확증득도적약270bp적편단진행분리화극륭,병수궤도선료40개극륭진행측서,결과득도료36개단독적핵산서렬,기중18개함유이마돌변혹종지자.근거서렬비대,저사극륭가분위9조이급단개적9충.저40개극륭중,핵산서렬상사성종8%도100%,현시출기핵산서렬적고도이질성.장저사극륭적핵산서렬여래자기타충식물적상응서렬진행보계분석,발현유사극륭여래자기타충식물적상응서렬적친연관계비저사극륭지간경위접근.반점잡교현시Ty1/copia반전록전좌자약점록두기인조적9.3%.
Ty1/copia-like sequences were amplified from mung bean ( Vigna radiata (L.) Wilczek) genomic DNA, by PCR with degenerate oligonucleotide primers corresponding to highly conserved domains in the Ty1/copia-like retrotransposons. PCR fragments of roughly 270 bp were isolated and cloned, and forty clones were sequenced. Thirty-six of the forty clones had unique nucleotide sequences, and eighteen clones had a frameshift, a stop codon, or both. Alignment of the nucleotide sequences indicated that these clones, denoted Tvr, fell into nine subgroups and nine ungrouped sequences. The nucleotide sequence similarity between these elements ranged from 8% to 100%, which indicates high level of sequence heterogeneity among these clones. A phylogenetic analysis comparing these clones with corresponding sequences from other plant species showed that some of the Tvr clones are more closely related to Ty1/copia-like retrotransposons from other species than to other Tvr clones. Dot blot analysis revealed that Ty1/copia-like retrotransposons comprise about 9.3% of the mung bean genome.