CAJ | 학술논문

学术背景:尽管一些实验已提出细胞膜可能是电磁生物效应的靶细胞部位之一的证据,但揭示电磁辐射细胞膜的通透性以及认识电磁辐射细胞生物效应的报道仍较少.目的:探讨低功率毫米波辐射与人白血病HL60细胞膜通透性及细胞凋亡的关系.设计:辐射组4组与非辐射组对照实验.单位:实验于2006-11/2007-04在解放军广州军区广州总医院医学实验中心实验室完成.材料:人白血病HL60细胞株由解放军广州军区医学实验中心提供,HD-413.2HPSG100毫米波辐射装置由西安恒达微波技术开发公司研制.方法:应用频率为41.32 GHz、平均功率密度为2 mW/cm2的毫米波辐射HL60细胞,根据辐射时间分为辐射0.15,30.45,60min组.主要观察指标:镧示踪法观察各组细胞内外镧颗粒分布,评价细胞膜通透性变性的变化;透射电镜观察超微结构细胞形态特点:原位标记法行细胞凋亡阳性定量分析.结果:①毫米波辐射HL60细胞45 min和60 min可见少量镧颗粒进入细胞质内.超微结构观察显示线粒体肿胀.粗面内质网扩张.细胞凋亡易见.②TUNEL法染色显示辐射15,30,45,60 min组凋亡率较0 min组有增加的趋势,尤以45 min和60 min组细胞凋亡明显增加.结论:低功率毫米波辐射HL60细胞,导致细胞膜通透性增加是促进细胞凋亡及产生系列生物学效应的重要因素之.
학술배경:진관일사실험이제출세포막가능시전자생물효응적파세포부위지일적증거,단게시전자복사세포막적통투성이급인식전자복사세포생물효응적보도잉교소.목적:탐토저공솔호미파복사여인백혈병HL60세포막통투성급세포조망적관계.설계:복사조4조여비복사조대조실험.단위:실험우2006-11/2007-04재해방군엄주군구엄주총의원의학실험중심실험실완성.재료:인백혈병HL60세포주유해방군엄주군구의학실험중심제공,HD-413.2HPSG100호미파복사장치유서안항체미파기술개발공사연제.방법:응용빈솔위41.32 GHz、평균공솔밀도위2 mW/cm2적호미파복사HL60세포,근거복사시간분위복사0.15,30.45,60min조.주요관찰지표:란시종법관찰각조세포내외란과립분포,평개세포막통투성변성적변화;투사전경관찰초미결구세포형태특점:원위표기법행세포조망양성정량분석.결과:①호미파복사HL60세포45 min화60 min가견소량란과립진입세포질내.초미결구관찰현시선립체종창.조면내질망확장.세포조망역견.②TUNEL법염색현시복사15,30,45,60 min조조망솔교0 min조유증가적추세,우이45 min화60 min조세포조망명현증가.결론:저공솔호미파복사HL60세포,도치세포막통투성증가시촉진세포조망급산생계렬생물학효응적중요인소지.
BACKGROUND: There are some studies proposing cell membrane may be one of target cell regions for electromagnetic biological effects. However, reports responsible for cellular membrane permeability and cellular biological effects after electromagnetic irradiation are few. OBJECTIVE: This study was designed to investigate the correlations of low-power millimeter wave irradiation to cellular membrane permeability and apoptosis of HL60.DESIGN: Controlled experiment.SETTING: Thus study was performed at the Medical Experimental Central Laboratory, Guangzhou General Hospital,Guangzhou Military Area Command of Chinese PLA between November 2006 and April 2007. MATERIALS: The human leukemic cell lines HL60 were kindly provided by Medical Experimental Center of Guangzhou Military Area Command of Chinese PLA and HD-413.2HPSG 100 millimeter wave irradiation generator was developed by Xi'an Hengda Microwave Technology Development Company, China. METHODS: HL60 cells were irradiated by millimeter wave at frequency of 41.32 GHz and mean power density of 2 mW/cm2, and divided into five groups according to the irradiation time (0, 15, 30, 45, 60 minutes groups).MAIN OUTCOME MEASURES: Lanthanum tracing was used to observe intracellular and extracellular lanthanum particles distribution and evaluate the change of cellular membrane permeability; ultrastructure and morphological characteristics of HL60 cells were observed through an transmission electron microscope; Quantitative analysis of apoptotic cells was performed by in situ marking method. RESULTS: In the 45 and 60 minutes groups, a small amount of lanthanum particles in the cytoplasm, swollen mitochondria, expanded rough endoplasmic reticulum, and obvious apoptosis were detected by ultrastructure observation. TUNEL staining showed, compared with the 0 minute control group, the apoptosis rate showed a trend of elevation in all the irradiation groups, particularly in the 45 and 60 minutes groups. CONCLUSION: Low-power millimeter wave irradiation on HL60 cells can lead to an increase in cellular membrane permeability of HL60, which may be one of the primary causes for promoting apoptosis and producing other biological effects.