CAJ | 학술논문

为深入了解四川小麦新品种(系)中抗条锈病基因的组成,并为开展分子标记辅助育种提供依据,以小麦抗条锈病基因Yr17的近等基因系以及抗条锈病品系L15与感条锈病材料SY95-71的杂交F2代群体为材料,采用集群分离分析法(BSA)结合SSR分子标记进行分析,发现1条由引物Xgwm415扩增的与抗条锈基因Yr17连锁的特异带.对该特异带克隆测序发现,该序列长390 bp,与栽培大麦中的一个序列表达标签(EST)有90%的相似性,与水稻基因组中的一个"核苷酸结合位点(NBS)-富亮氨酸重复(LRR)"型抗病基因的编码区有70%的相似性.根据该序列设计特异引物,建立SCAR标记,命名为SC-372.利用SC-372对54个四川省近年育成的小麦品种(系)进行检测,发现在15个抗病品种(系)中能特异扩增.进一步利用SC-372及前人报道的Yr17连锁标记VENTRUP/LN2对Yr17近等基因系及偏凸山羊草进行扩增比较发现,本实验建立的SCAR标记对小麦背景中的Yr17基因有更好的检测效率.因此可将SC-372用于分子标记聚合小麦抗条锈病基因的育种实践中.
위심입료해사천소맥신품충(계)중항조수병기인적조성,병위개전분자표기보조육충제공의거,이소맥항조수병기인Yr17적근등기인계이급항조수병품계L15여감조수병재료SY95-71적잡교F2대군체위재료,채용집군분리분석법(BSA)결합SSR분자표기진행분석,발현1조유인물Xgwm415확증적여항조수기인Yr17련쇄적특이대.대해특이대극륭측서발현,해서렬장390 bp,여재배대맥중적일개서렬표체표첨(EST)유90%적상사성,여수도기인조중적일개"핵감산결합위점(NBS)-부량안산중복(LRR)"형항병기인적편마구유70%적상사성.근거해서렬설계특이인물,건립SCAR표기,명명위SC-372.이용SC-372대54개사천성근년육성적소맥품충(계)진행검측,발현재15개항병품충(계)중능특이확증.진일보이용SC-372급전인보도적Yr17련쇄표기VENTRUP/LN2대Yr17근등기인계급편철산양초진행확증비교발현,본실험건립적SCAR표기대소맥배경중적Yr17기인유경호적검측효솔.인차가장SC-372용우분자표기취합소맥항조수병기인적육충실천중.
To further clarify the composition of stripe rust resistance gene in the newly developed wheat cultivars (lines) of Sichuan and establish molecular marker-assisted breeding system for rust resistance, the Thatcher Yr17 near isogeneic lines (NILs) and a F2 population derived from a cross between developed resistant line L15 and susceptible line SY95-71 were used to screen the SSR markers by bulked segregation analysis (BSA). A PCR product amplified by Xgwm415 was linked to resistance gene to strip rust resistance gene Yr17. The 390bp sequence of the PCR fragment had 90% identity to one EST of barley and 70% identity to the CDS of a NBS-LRR disease resistance protein in rice. Basing on this sequence, a pair of sequence-characterized amplified region (SCAR) primers were designed, and the SCAR marker, named SC-372, was able to target 15 resistant cultivars (lines) in all 54 wheat cultivars of newly developed in Sichuan Province. Compared with the amplification of marker VENTRUP/LN2 linked to Yr17, the SC-372 marker showed a higher detection efficiency to Yr17 when a set of NILs and Aegilops ventricosa were amplified. It was therefore to conclude that the new SCAR marker for Yr17 gene and the identified resistant lines could be used in wheat breeding for stripe rust resistance.