CAJ | 학술논문

随机采取无直接亲缘关系的68只鸵鸟血样,采用微卫星标记技术对鸵鸟20个微卫星座位进行遗传多样性检测.结果表明,有3个座位(CAU13、CAU36、CAU46)没有多态性,其他17个座位(LEI0094、CAU5、CAU7、CAU8、CAU11、CAU14、CAU15、CAU16、CAU17、CAU22、CAU23、CAU24、CAU32、CAU34、CAU42、CAU43、和CAU57)共检测到142个等位基因,平均每个座位上等位基因数为8.3529±1.2803,平均期望杂合度及多态信息含量分别为0.8468±0.1368和0.8213±0.0321.鸵鸟群体内存在丰富的遗传变异,遗传多样性较高.
수궤채취무직접친연관계적68지타조혈양,채용미위성표기기술대타조20개미위성좌위진행유전다양성검측.결과표명,유3개좌위(CAU13、CAU36、CAU46)몰유다태성,기타17개좌위(LEI0094、CAU5、CAU7、CAU8、CAU11、CAU14、CAU15、CAU16、CAU17、CAU22、CAU23、CAU24、CAU32、CAU34、CAU42、CAU43、화CAU57)공검측도142개등위기인,평균매개좌위상등위기인수위8.3529±1.2803,평균기망잡합도급다태신식함량분별위0.8468±0.1368화0.8213±0.0321.타조군체내존재봉부적유전변이,유전다양성교고.
In order to study ostrich's genetic characteristics on the molecular level,68 individuals without direct relationship were picked out according to the random sampling method. The genetic diversity was analyzed using 20 microsatellite markers. The results showed that a total of 142 alleles were detected in 17 microsatellite loci (LEI0094,CAU5,CAU7,CAU8,CAU11,CAU14,CAU15,CAU16,CAU17,CAU22,CAU23,CAU24,CAU32,CAU34,CAU42,CAU43 and CAU57 ),excepting 3 non-polymorphic microsatellite loci (CAU13,CAU36,CAU46),the number of alleles per locus was 8.3529±1.2803,the average expected heterozygosity and the average value of polymorphism information content(PIC)were 0.8468±0.1368 and 0.8213±0.0321 respectively. These results indicated that there were abundant genetic variations in this ostrich group,the genetic diversity was very high.