CAJ | 학술논문

目的探讨CD4 - CD8 - DNT细胞对体内、外胰腺癌生长的抑制作用.方法采用四甲基偶氮唑盐法检测DNT细胞对人胰腺癌细胞株Panc-1生长的影响.将18只裸鼠随机分成3组,其中前两组皮下注射Panc-1细胞以建立荷瘤鼠人胰腺癌皮下移植瘤模型,第3组注射DNT与Panc-1细胞按数量5∶1共培养后的细胞混悬液.前两组待成瘤后,再随机分为对照组和治疗组.治疗组予塞来昔布4 mg/d口服,对照组每日给予等量蒸馏水口服.每2周测量裸鼠肿瘤体积一次,描绘肿瘤生长曲线.治疗结束后处死裸鼠,剥除瘤组织,测量肿瘤体积并计算抑瘤率.结果 (1)四甲基偶氮唑盐结果显示,DNT细胞能在体外抑制人胰腺癌细胞株Panc-1的生长,且DNT细胞对胰腺癌细胞的生长抑制呈剂量依赖性；(2) DNT细胞能够干预人胰腺癌裸鼠皮下移植瘤的生长.结论 DNT细胞能够抑制体内、外胰腺癌细胞的生长.
목적 탐토CD4 - CD8 - DNT세포대체내、외이선암생장적억제작용.방법 채용사갑기우담서염법검측DNT세포대인이선암세포주Panc-1생장적영향.장18지라서수궤분성3조,기중전량조피하주사Panc-1세포이건립하류서인이선암피하이식류모형,제3조주사DNT여Panc-1세포안수량5∶1공배양후적세포혼현액.전량조대성류후,재수궤분위대조조화치료조.치료조여새래석포4 mg/d구복,대조조매일급여등량증류수구복.매2주측량라서종류체적일차,묘회종류생장곡선.치료결속후처사라서,박제류조직,측량종류체적병계산억류솔.결과 (1)사갑기우담서염결과현시,DNT세포능재체외억제인이선암세포주Panc-1적생장,차DNT세포대이선암세포적생장억제정제량의뢰성；(2) DNT세포능구간예인이선암라서피하이식류적생장.결론 DNT세포능구억제체내、외이선암세포적생장.
Objective To find out the inhibitory effects of CD4 - CD8 - DNT cells on growth of which depresses the pancreatic cancer in vitro and in vivo.Methods The inhibitory effects of DNT cells on the growth of Panc- 1 were studied in vitro by MTT method.Eighteen BALB/c mice were divided into 3 groups randomly.Human pancreatic cancer xenografts were established in 2 groups randomly.The last group was injected the cell suspension which comprises DNT and Panc- 1 cells ( Panc- 1∶ DNT =1∶ 5 ).When the diameter of tumor was about 5 mm,the first 2 group mice were further divided into 2 groups randomly.One was control,treated with distilled water.The other was treated with celebrex (4 mg/d).The size of the tumors was calculated every 2 weeks and tumor growth curve was depicted.At the end of the treatments,the mice were sacrific and the tumors were harvested.The tumor inhibition rate was calculated.Results ( 1 ) MTT study showed that DNT cells produced a dose- dependent inhibition of Panc- 1 proliferation in vitro.(2) The growth of transplanted pancreatic cancer was down-regulated by treatment of DNT cells.Conclusion DNT cells can inhibit the growth of pancreatic cancer in vitro and in vivo.