CAJ | 학술논문

目的探讨有色金属锡粉尘导致实验动物肺损伤是否具有锡尘肺的特异性病变,为诊断锡尘肺提供佐证.方法选择SPF级成年SD大鼠40只,进行锡粉尘染尘动物实验,每组10只,随机分为锡粗炼车间粉尘组、锡精炼车间粉尘组、标准石英粉尘阳性对照组(用93％的标准石英粉尘染尘)和生理盐水阴性对照组,随后动态观察大鼠肺的病理组织学变化.结果实验动物在染锡粉尘后第30天眼观可见两肺的表面和切面有散在的灰白色针尖大小斑点,光学显微镜下见在细小支气管周围散在分布的局灶性肉芽肿性炎,较多粉尘颗粒沉着;第90天肉芽肿性炎加重,并见明显的成纤维细胞增生和胶原纤维形成,VG染色阳性;基本达到锡尘肺早期特异性病变的诊断标准.结论证实有色金属锡粉尘可以导致大鼠实验染尘后发生特异性肺损伤,即肺组织出现的肉芽肿病变,达到锡尘肺早期特异性病变的病理诊断标准.
목적 탐토유색금속석분진도치실험동물폐손상시부구유석진폐적특이성병변,위진단석진폐제공좌증.방법 선택SPF급성년SD대서40지,진행석분진염진동물실험,매조10지,수궤분위석조련차간분진조、석정련차간분진조、표준석영분진양성대조조(용93％적표준석영분진염진)화생리염수음성대조조,수후동태관찰대서폐적병리조직학변화.결과 실험동물재염석분진후제30천안관가견량폐적표면화절면유산재적회백색침첨대소반점,광학현미경하견재세소지기관주위산재분포적국조성육아종성염,교다분진과립침착;제90천육아종성염가중,병견명현적성섬유세포증생화효원섬유형성,VG염색양성;기본체도석진폐조기특이성병변적진단표준.결론 증실유색금속석분진가이도치대서실험염진후발생특이성폐손상,즉폐조직출현적육아종병변,체도석진폐조기특이성병변적병리진단표준.
Objective To explore the characters of lung injury induced by tin dusts and to provide the diagnosis evidence of tin pneumoconiosis.Methods Forty SD rats were randomly divided into four groups:the group exposed to tin dusts from smelting workshop,the group exposed to tin dusts from tin refining workshop,the positive control group exposed to standard quartz dusts and the negative control group exposed to saline.The pathological changes of rat lungs were observed dynamically.Results In rats exposed to tin dusts,on the 30th day after exposure to tin dusts,the scattered hoar tip size of the spots in surface and section of the lungs were observed,the scattered focal granulomatous inflammation around the small bronchi and dust particles in lung tissue were observed under microscope; on the 90th day after exposure to tin dusts,the granulomatous inflammation increase,the fibroblasts proliferation,collagen fibers formation and positive VG staining were found.There were significant differences,as compared with positive or negative controls (P＜0.05).These pathological changes were basically the characters of specific pathological changes in early tin pneumoconiosis.Conclusion Non-ferrous metal tin dusts can induce the specific lung injury (granuloma formation) in lung tissue of rats exposed to tin dusts,which fulfilled the diagnostic criteria of specific pathological changes in early tin pneumoconiosis.