中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
4期
277-279
,共3页
李翠梅%张淑杰%朱建华%韩光亮
李翠梅%張淑傑%硃建華%韓光亮
리취매%장숙걸%주건화%한광량
肺肿瘤%爱泼斯坦巴尔病毒感染%病毒包膜蛋白质类%基因
肺腫瘤%愛潑斯坦巴爾病毒感染%病毒包膜蛋白質類%基因
폐종류%애발사탄파이병독감염%병독포막단백질류%기인
Lung neoplasms%Epstein Barr Virus infections%Viral envelope proteins%Genes
目的 研究肺癌患者癌组织中EB病毒(Epstein-Barr virus，EBV)感染、EBV潜伏膜蛋白1 (latent membrane protein 1，LMP1)和B淋巴细胞瘤/白血病-2基因(B cell lymphoma/Leukemia-2,Bcl-2)的表达及意义。方法 采用原位杂交法（in situ hybridization，ISH）检测肺癌组织标本中EBV编码的小RNA( EBER1)，免疫组化法(immunohistochemistry，IHC)检测Bcl-2和LMP1的表达，以GD-6多媒体彩色病理图像分析系统进行形态学定量，以图像的平均面积(average area，AA)和积分吸光度（Integral optical density，IA）表示表达量的多少。结果 在108例肺癌癌组织中36例EBV阳性，阳性率为33.3％；LMP1阳性表达7例，阳性率6.5％。EBV阳性肺癌组中Bcl-2表达显著高于EBV阴性组，其AA分别为58014.23±6918.45和38156.22±4096.79，其IA分别为11.00±1.48和8.03±0.78,差异有统计学意义。进一步分析LMPI和Bcl-2表达的关系，可见LMP1可使Bcl-2表达率增加，但定性分析和定量分析差异无统计学意义。结论 EBV感染使Bcl-2表达增加，可能在肺癌的发生发展中有一定作用。在肺癌组织中EBV可能不是通过LMP1来影响Bcl-2的表达，具体机制有待进一步研究。
目的 研究肺癌患者癌組織中EB病毒(Epstein-Barr virus，EBV)感染、EBV潛伏膜蛋白1 (latent membrane protein 1，LMP1)和B淋巴細胞瘤/白血病-2基因(B cell lymphoma/Leukemia-2,Bcl-2)的錶達及意義。方法 採用原位雜交法（in situ hybridization，ISH）檢測肺癌組織標本中EBV編碼的小RNA( EBER1)，免疫組化法(immunohistochemistry，IHC)檢測Bcl-2和LMP1的錶達，以GD-6多媒體綵色病理圖像分析繫統進行形態學定量，以圖像的平均麵積(average area，AA)和積分吸光度（Integral optical density，IA）錶示錶達量的多少。結果 在108例肺癌癌組織中36例EBV暘性，暘性率為33.3％；LMP1暘性錶達7例，暘性率6.5％。EBV暘性肺癌組中Bcl-2錶達顯著高于EBV陰性組，其AA分彆為58014.23±6918.45和38156.22±4096.79，其IA分彆為11.00±1.48和8.03±0.78,差異有統計學意義。進一步分析LMPI和Bcl-2錶達的關繫，可見LMP1可使Bcl-2錶達率增加，但定性分析和定量分析差異無統計學意義。結論 EBV感染使Bcl-2錶達增加，可能在肺癌的髮生髮展中有一定作用。在肺癌組織中EBV可能不是通過LMP1來影響Bcl-2的錶達，具體機製有待進一步研究。
목적 연구폐암환자암조직중EB병독(Epstein-Barr virus，EBV)감염、EBV잠복막단백1 (latent membrane protein 1，LMP1)화B림파세포류/백혈병-2기인(B cell lymphoma/Leukemia-2,Bcl-2)적표체급의의。방법 채용원위잡교법（in situ hybridization，ISH）검측폐암조직표본중EBV편마적소RNA( EBER1)，면역조화법(immunohistochemistry，IHC)검측Bcl-2화LMP1적표체，이GD-6다매체채색병리도상분석계통진행형태학정량，이도상적평균면적(average area，AA)화적분흡광도（Integral optical density，IA）표시표체량적다소。결과 재108례폐암암조직중36례EBV양성，양성솔위33.3％；LMP1양성표체7례，양성솔6.5％。EBV양성폐암조중Bcl-2표체현저고우EBV음성조，기AA분별위58014.23±6918.45화38156.22±4096.79，기IA분별위11.00±1.48화8.03±0.78,차이유통계학의의。진일보분석LMPI화Bcl-2표체적관계，가견LMP1가사Bcl-2표체솔증가，단정성분석화정량분석차이무통계학의의。결론 EBV감염사Bcl-2표체증가，가능재폐암적발생발전중유일정작용。재폐암조직중EBV가능불시통과LMP1래영향Bcl-2적표체，구체궤제유대진일보연구。
Objective To investigate the Epstein-Barr virus (EBV) infection, the expression of EBV latent membrane protein 1 ( LMP1 ) and oncogene bcl-2 in lung cancer patients. Methods EBER1 in 108 cases of lung cancer were detected with in situ hybridization. EBV positive and negative lung cancer tissues were analysed for the expression of LMP1 and Bcl-2 by immnohistochemistry. The average area (AA)and integral optical density (IA) of each sample was measured with the digital medical image analyzing system. ResultsIn 108 cases of lung cancer, 36 cases were EBER1 positive and 7 cases were LMP1positive. The expression of Bcl-2 was higher in EBV positive lung cancer tissues than that in EBV negative.The AA value was 58014. 23 ±6918.45 and 38156.22 ± 4096. 79, while the IA value was 11. 00 ± 1.48 and 8.03 ±0. 78 respectively. No statistic difference was fund in the expression of Bcl-2 betwen LMP1 positive and negative lung cancer tisssues. ConclusionEBV infection in lung cancer increased the expression of bcl-2, which may play a role in the occurrence or development of lung cancer. The increased expression of Bcl-2 may not be induced by LMP1. The exact mechanism need further study.
