中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
18期
1276-1280
,共5页
赵京玉%柴家科%宋慧锋%孙天骏%李东杰%刘玲英%高全文%梁黎明
趙京玉%柴傢科%宋慧鋒%孫天駿%李東傑%劉玲英%高全文%樑黎明
조경옥%시가과%송혜봉%손천준%리동걸%류령영%고전문%량려명
透明质酸%移植,异种%皮肤移植%胶原蛋白%脱细胞真皮基质
透明質痠%移植,異種%皮膚移植%膠原蛋白%脫細胞真皮基質
투명질산%이식,이충%피부이식%효원단백%탈세포진피기질
Hyaluronic acid%Transplantation,heterologous%Skin transplantation%Collagen%Acellular dermal matrix
目的 探讨外源性透明质酸(HA)对异种(猪)脱细胞真皮基质(PADM)复合移植后组织中Ⅰ、Ⅲ型胶原蛋白的影响.方法 以30只日本大耳白兔为实验动物,在其背部制作伞层皮肤缺损动物模型,随机分为HA+PADM+薄自体皮移植组(A组)、PADM+薄自体皮移植组(B组)和薄自体皮移植组(C组)3组.术后50 d取材,采用病理学、免疫组化、Western印迹等方法检测Ⅰ、Ⅲ型胶原蛋白的变化并进行统计学对比分析.结果 A、B、C组植皮后面积分别为(13.3 4±1.2)、(9.5.4±0.9)、(10.0±1.4)cm2,A组明显大于B组和C组(P<0.01),B组和C组相比差异无统计学意义(P>0.05).3组Ⅰ型胶原蛋白表达分别为1894±164、515±38、395±43,A组明显高于B、C组(均P<0.01);m型胶原蛋白表达分别为5411±435、874±70、2078±175,A组>C组>B组(均P<0.01);Ⅰ/Ⅲ型胶原比例分别为0.35、0.59、0.19,A、B组均明显高于c组(均P<0.01).结论 外源性HA能够增加复合皮肤移植后Ⅰ、Ⅲ型胶原蛋白表达,降低Ⅰ/Ⅲ型胶原蛋白比例,有利于复合皮移植后真皮和基底膜的重塑,有助于减轻挛缩,改善复合皮移植质量.
目的 探討外源性透明質痠(HA)對異種(豬)脫細胞真皮基質(PADM)複閤移植後組織中Ⅰ、Ⅲ型膠原蛋白的影響.方法 以30隻日本大耳白兔為實驗動物,在其揹部製作傘層皮膚缺損動物模型,隨機分為HA+PADM+薄自體皮移植組(A組)、PADM+薄自體皮移植組(B組)和薄自體皮移植組(C組)3組.術後50 d取材,採用病理學、免疫組化、Western印跡等方法檢測Ⅰ、Ⅲ型膠原蛋白的變化併進行統計學對比分析.結果 A、B、C組植皮後麵積分彆為(13.3 4±1.2)、(9.5.4±0.9)、(10.0±1.4)cm2,A組明顯大于B組和C組(P<0.01),B組和C組相比差異無統計學意義(P>0.05).3組Ⅰ型膠原蛋白錶達分彆為1894±164、515±38、395±43,A組明顯高于B、C組(均P<0.01);m型膠原蛋白錶達分彆為5411±435、874±70、2078±175,A組>C組>B組(均P<0.01);Ⅰ/Ⅲ型膠原比例分彆為0.35、0.59、0.19,A、B組均明顯高于c組(均P<0.01).結論 外源性HA能夠增加複閤皮膚移植後Ⅰ、Ⅲ型膠原蛋白錶達,降低Ⅰ/Ⅲ型膠原蛋白比例,有利于複閤皮移植後真皮和基底膜的重塑,有助于減輕攣縮,改善複閤皮移植質量.
목적 탐토외원성투명질산(HA)대이충(저)탈세포진피기질(PADM)복합이식후조직중Ⅰ、Ⅲ형효원단백적영향.방법 이30지일본대이백토위실험동물,재기배부제작산층피부결손동물모형,수궤분위HA+PADM+박자체피이식조(A조)、PADM+박자체피이식조(B조)화박자체피이식조(C조)3조.술후50 d취재,채용병이학、면역조화、Western인적등방법검측Ⅰ、Ⅲ형효원단백적변화병진행통계학대비분석.결과 A、B、C조식피후면적분별위(13.3 4±1.2)、(9.5.4±0.9)、(10.0±1.4)cm2,A조명현대우B조화C조(P<0.01),B조화C조상비차이무통계학의의(P>0.05).3조Ⅰ형효원단백표체분별위1894±164、515±38、395±43,A조명현고우B、C조(균P<0.01);m형효원단백표체분별위5411±435、874±70、2078±175,A조>C조>B조(균P<0.01);Ⅰ/Ⅲ형효원비례분별위0.35、0.59、0.19,A、B조균명현고우c조(균P<0.01).결론 외원성HA능구증가복합피부이식후Ⅰ、Ⅲ형효원단백표체,강저Ⅰ/Ⅲ형효원단백비례,유리우복합피이식후진피화기저막적중소,유조우감경련축,개선복합피이식질량.
Objective To investigate the changes of collagens Ⅰ and Ⅲ after the addition of hyaluronic acid in the transplantation of porcine acellular dermal matrix.Methods Full-thickness skin defects were created on the dorsa of Japanese white rabbits.And the rabbits were divided randomly into 3 groups:Group A(hyaluronic acid,porcine acellular dermal matrix plus thin skin autografts),Group B (porcine acellular dermal matrix plus thin skin autografts)and Group C (skin autografts).Skin biopsies were performed at Day 50 post-grafting to detect the contents of collagens Ⅰ and Ⅲ by histological examinations,immunohistochemistry method and Western blot.Results The areas of skin graft were(13.34±1.2),(9.5±0.9)and(10.0±1.4)cm2 in Groups A,B and C respectively.Group A was larger than Groups B and C(all P<0.01).There was no statistical difference between Groups B and C(P>0.05).The expressions of collagen Ⅰ were 1894±164,515±38 and 395±43 in Groups A,B and C respectively.Group A was higher than Groups B and C(P<0.01).And the expressions of collagen Ⅲ were 5411±435,874±70 and 2078±175 in Groups A,B and C respectively.Group C was higher than Group B and yet lower than Group A(all P<0.01).The ratios of collagen Ⅰ and collagen Ⅲ in Group A(0.39)and Group B(0.59)were higher than that of Group C(O.19)(all P<0.01).Conclusion The addition of hyaluronic acid may boost the expression of collagens Ⅰ andⅢand decrease the ratio of collagen Ⅰ/collagenⅢ.Thus it facilitates wound healing and basilar membrane remodeling and alleviates the contraction of skin transplant.
