分析科学学报
分析科學學報
분석과학학보
JOURNAL OF ANALYTICAL SCIENCE
2009年
5期
590-592
,共3页
2-(9-蒽基)-2-甲氧基乙酸乙酯%高效液相色谱法%手性分离
2-(9-蒽基)-2-甲氧基乙痠乙酯%高效液相色譜法%手性分離
2-(9-은기)-2-갑양기을산을지%고효액상색보법%수성분리
(±)-Ethyl 2-(9-anthryl)-2-methoxyacetate%High-performance liquid chromatography%Chiral resolution
以键合在5 μm硅胶上的纤维素-三(二甲基苯基氨基甲酸酯)为色谱柱的手性固定相,采用高效液相色谱(HPLC)法对外消旋的2-(9-蒽基)-2-甲氧基乙酸乙酯进行了手性拆分.对影响2-(9-蒽基)-2-甲氧基乙酸乙酯拆分的三个重要因素:流动相组成、流速、色谱柱温度进行了研究.实验结果表明,在流动相组成为正已烷-异丙醇(94/6,V/V),流速1.0 mL/min,柱温20℃的条件下,2-(9-蒽基)-2-甲氧基乙酸乙酯对映体得到很好的分离,分离度为3.63.
以鍵閤在5 μm硅膠上的纖維素-三(二甲基苯基氨基甲痠酯)為色譜柱的手性固定相,採用高效液相色譜(HPLC)法對外消鏇的2-(9-蒽基)-2-甲氧基乙痠乙酯進行瞭手性拆分.對影響2-(9-蒽基)-2-甲氧基乙痠乙酯拆分的三箇重要因素:流動相組成、流速、色譜柱溫度進行瞭研究.實驗結果錶明,在流動相組成為正已烷-異丙醇(94/6,V/V),流速1.0 mL/min,柱溫20℃的條件下,2-(9-蒽基)-2-甲氧基乙痠乙酯對映體得到很好的分離,分離度為3.63.
이건합재5 μm규효상적섬유소-삼(이갑기분기안기갑산지)위색보주적수성고정상,채용고효액상색보(HPLC)법대외소선적2-(9-은기)-2-갑양기을산을지진행료수성탁분.대영향2-(9-은기)-2-갑양기을산을지탁분적삼개중요인소:류동상조성、류속、색보주온도진행료연구.실험결과표명,재류동상조성위정이완-이병순(94/6,V/V),류속1.0 mL/min,주온20℃적조건하,2-(9-은기)-2-갑양기을산을지대영체득도흔호적분리,분리도위3.63.
The racemic ethyl 2-(9-anthryl)-2-methoxyacetate was resoluted by high-performance liquid chromatography with a cellulose-tris-3,5-dimethylphenyl carbamate-based chiral column successfully. Various factors which can affect the resolution of the compound such as mobile phase, flow rate and column temperature were investigated. Under the conditions of 6% of 2-propanol in n-hexane (V/V) as mobile phase, flow rate of 1.0 mL/min and column temperature of 20℃, (±)-9AMA were successfully separated at Rs 3.63.
