CAJ | 학술논문

以杜×(大×长)三元杂种猪为研究对象,将其分为2组,分别饲喂基础日粮和添加1%精氨酸日粮.测定试验猪血液白细胞分类计数,血清免疫球蛋白和细胞因子水平、淋巴细胞增殖活性以及脾脏IL-2、TNF-α和IFN-γ的mRNA表达水平,探讨日粮添加精氨酸对肥育猪免疫功能的调节作用.结果表明:日粮中添加1%精氨酸可显著增加单核细胞数和百分比、淋巴细胞百分比和增殖活性及血清IgG和IL-2水平,显著降低中性粒细胞数和百分比及血清TNF-α水平;明显增加脾脏IL-2和IFN-γ表达水平,降低TNF-α表达水平.结果提示,精氨酸可通过调节肥育猪的细胞和体液免疫水平,从而增强机体免疫功能.
이두×(대×장)삼원잡충저위연구대상,장기분위2조,분별사위기출일량화첨가1%정안산일량.측정시험저혈액백세포분류계수,혈청면역구단백화세포인자수평、림파세포증식활성이급비장IL-2、TNF-α화IFN-γ적mRNA표체수평,탐토일량첨가정안산대비육저면역공능적조절작용.결과표명:일량중첨가1%정안산가현저증가단핵세포수화백분비、림파세포백분비화증식활성급혈청IgG화IL-2수평,현저강저중성립세포수화백분비급혈청TNF-α수평;명현증가비장IL-2화IFN-γ표체수평,강저TNF-α표체수평.결과제시,정안산가통과조절비육저적세포화체액면역수평,종이증강궤체면역공능.
This study was conducted to evaluate the effects of arginine on immune response in fattening pigs.Twenty Duroc×Landrace× Yorkshire pigs with about 50 kg of body weight were randomly assigned to 2 treatment groups(10pigs per group)representing the addition of 0 or 1% arginine to basal diets.On 60 days after initiation of the addition,total and differential counts of leucocytes,serum levels of immunoglobulins(Ig)and cytokines,and proliferation activity of peripheral lymphocytes were determined,as well as mRNA expression levels of IL-2,TNF-α and IFN-γ in spleen.The results showed that dietary supplementation with 1% arginine increased the monocyte number,percentages of monoeyte and lymphocyte,proliferation activity of lymphocyte,and serum contents of IgG and IL-2(P<0.05),as well as gene expression of IL-2 and IFN-γ(P>0.05),and decreased the number and percentage of neutrophils,and serum content of TNF-α(P<0.05),as well as expression level of TNF-α(P>0.05)in comparison with the control group.In conclusion,these findings suggest that the 1% arginine as a dietary additive enhances the immune function of fattening pigs by modulating the cellular and humoral immune responses.