CAJ | 학술논문

花色是重要的园艺性状,一直是育种工作者苦苦追求的目标.利用植物基因工程技术可定向改良花色.根据已知的CHS序列(序列号M20308.),用PCR方法从拟南芥中克隆CHS基因,并分别将其以正向、反向插入到真核表达载体pBI121,在农杆菌介导下用叶盘转化法转化烟草.对转基因烟草进行检测,结果表明,转基因烟草的花色变淡、花青素含量降低;叶片颜色变浅、叶绿素含量降低.转基因烟草花的形态也发生了明显变异.
화색시중요적완예성상,일직시육충공작자고고추구적목표.이용식물기인공정기술가정향개량화색.근거이지적CHS서렬(서렬호M20308.),용PCR방법종의남개중극륭CHS기인,병분별장기이정향、반향삽입도진핵표체재체pBI121,재농간균개도하용협반전화법전화연초.대전기인연초진행검측,결과표명,전기인연초적화색변담、화청소함량강저;협편안색변천、협록소함량강저.전기인연초화적형태야발생료명현변이.
Flower colour mainly determined by anthocyanins is an important trait.The floral color could be modified by using engineering technology such as antisence,co-supression,RNAi and multipal gene regulation.According to the submitted chs sequence,we cloned CHS gene from Arabidopsis thaliana by PCR method.The CHS gene was cloned into pBI121,and two expression vectors including pBI chs S (35S promoter:sence CHS gene)and pBI chsAN (35S promoter:antisence CHS gene) were obtained.Plant expression vectors were transformed into tobacco mediated by Agrobacterium LBA4404.Results indicated that both leaves colour and flower colour of transgenic tobacco was weaker than wild tobacco and Chlorophyll level and anthocyanin level was lower than that of the wild.It proved that not only flower colours of the transgenic plant were altered,but the flower mophology were modified too.