CAJ | 학술논문

目的探讨转化生长因子(TGF)-β_1基因L10P多态与结直肠癌(CRC)遗传易感性的关系.方法采用TaqMan方法检测345例CRC患者及670名对照者的TGF-β_1 L10P基因型的分布.结果两组间TGF-β_1 L10P基因型分布的差异无统计学意义(P=0.213).TNM分层分析结果显示,P是TNM Ⅰ～Ⅱ期CRC发病风险的遗传保护因子,与LL纯合子相比,LP杂合子的CRC发病风险显著降低至0.65(95%CI为0.44～0.95,P=0.027),PP纯合子的CRC发病风险显著降低至0.47(95%CI为0.28～0.78,P=0.004);将LP和PP基因型合并计算,则其CRC发病风险与LL基因型相比,降低至0.59(95%CI为0.41～0.85,P=0.005).结论 TGF-β_1基因L10P多态可能是中国人群TNM Ⅰ～Ⅱ期CRC发病风险的一个遗传保护因素.
목적 탐토전화생장인자(TGF)-β_1기인L10P다태여결직장암(CRC)유전역감성적관계.방법 채용TaqMan방법검측345례CRC환자급670명대조자적TGF-β_1 L10P기인형적분포.결과 량조간TGF-β_1 L10P기인형분포적차이무통계학의의(P=0.213).TNM분층분석결과현시,P시TNM Ⅰ～Ⅱ기CRC발병풍험적유전보호인자,여LL순합자상비,LP잡합자적CRC발병풍험현저강저지0.65(95%CI위0.44～0.95,P=0.027),PP순합자적CRC발병풍험현저강저지0.47(95%CI위0.28～0.78,P=0.004);장LP화PP기인형합병계산,칙기CRC발병풍험여LL기인형상비,강저지0.59(95%CI위0.41～0.85,P=0.005).결론 TGF-β_1기인L10P다태가능시중국인군TNM Ⅰ～Ⅱ기CRC발병풍험적일개유전보호인소.
Objective To investigate the association of the L10P polymorphism of transforming growth factor-beta 1 (TGF-β_1) gene and the genetic susceptibility to colorectal cancer (CRC) in a Chinese population. Methods The TGF-β_1 L10P genotypes were determined by using the real-time PCR with TaqMan assay in 345 CRC patients and 670 sex-, age-matched controls. Odds ratios (Ors) for CRC and 95% confidence intervals (Cis) from unconditional logistic regression models were used to evaluate relative risks. Potential CRC risk factors, including sex, age, cigarette smoking, alcohol drinking, body mass index (BMI) and family history of colorectal cancer were included in the logistic regression models as a covariate in the multivariate analyses on genotype and CRC risk. Results The distribution of TGF-β_1 L10P genotypes was not significantly different between the two groups (P = 0. 213). However, the P allele was significantly associated with a decreased risk for TNM stage Ⅰ-Ⅱ CRC as compared to the L allele. Compared with LL genotype, LP, PP and P-allele carriers (LP or PP genotypes) had a 0.65-fold (95% CI, 0.44-0.95, P = 0. 027), a 0.47-fold (95% CI, 0.28-0.78, P = 0.004), and a 0.59-fold (95% CI: 0.41-0.85, P= 0.005) decrease in risk of TNM stage Ⅰ-Ⅱ CRC, respectively. Conclusion The L10P polymorphism of the TGF-β_1 gene may has a protective effect against TNM stage Ⅰ-Ⅱ CRC in Chinese population.